Chlamydia IgG ELISA Kit

Sample

serum or plasma

Detection Method

iELISA

Intended Use

Chlamydia IgG ELISA is quantitative and qualitative tests for detection of human antibodies in serum or plasma directed against genus specific human Chlamydia-antibodies. For sale in the U.S. for Research Use Only. Not for use in diagnostic procedures.

Storage

1. microtiter strips (antigen), after opening at 2-8°C in closed aluminum bag with desiccant. Strips which are not used must be stored in the press-seal bag of aluminum compound foil under dry and airtight conditions! 4 weeks
2. control sera /standard sera, after opening at 2-8°C, until expiry date; 24 months from date of production
3. conjugate, ready-to-use solution, at 2-8°C, Avoid contamination (sterile tips!). until expiry date 28 months from date of production
4. dilution buffer, after opening at 2-8°C(24 months). Discard cloudy solutions! unopened (until expiry date; 36 months from date of production)
5. washing solution, concentrate after opening at 2-8°C (until expiry date), working dilution at 2-8°C (2 weeks), working dilution at room temperature (1 weeks). Bottles used for the working dilution should be cleaned regularly, discard cloudy solutions.
6. substrate, ready-to-use solution at 2-8°C, protected from light! Avoid contamination (sterile tips!) Discard when solution turns yellow (extinction against distilled water > 0.25). Until expiry date 24 months from date of production
7.stopping solution, after opening at room temperature, until expiry date

General Description

The order Chlamydiales currently has one family, the Chlamydiaceae, containing two genera, Chlamydia and Chlamydophila. Three human pathogenic species, Chlamydia trachomatis, Chlamydophila pneumoniae and Chlamydophila psittaci are belonging to these genera. Nearly all birds and mammals can be infected with C. psittaci, whereas man is the main host of C. trachomatis. Workers on poultry farms are in addition at high risk of C. psittaci infection which may cause a severe psittacosis disease. The different Chlamydia species are transmitted via different routes of infection: C. pneumoniae via aerosol transmission, C. trachomatis via direct contact or smear infection, C. psittaci via inhalation of fecal droppings, feather dust or smear infection. Chlamydia are coccoide, gram-negative bacterial pathogens which are metabolically deficient in their ability to synthesize ATP and thus have an obligate intracellular life cycle. During an infection with Chlamydia two characteristic cell forms occur: the highly infectious elementary bodies and the non-infectious intracellular reticular bodies which are able to propagate.