Chicken Syndecan 4 (SDC4) ELISA Kit

Sample

Serum, plasma, culture supernatants, or other biological fluids

Species Reactivity

Chicken

Detection Method

sELISA

Intended Use

The Chicken Syndecan 4 (SDC4) ELISA Kit is designed for the quantitative determination of SDC4 concentrations in chicken samples such as serum, plasma, culture supernatants, or other biological fluids.

Contents of Kit

1. Kit Equilibration
Allow the ELISA kit to reach room temperature before use.
2. Wash Buffer Preparation
Dilute the concentrated wash buffer with double-distilled water (1:25). Return any unused portion to storage.
3. Standard Reconstitution
Add 1.0 mL of Standard Diluent to the vial containing the lyophilized standard.
Let it stand for 30 minutes until fully dissolved, then mix gently and label as Tube No.0.
Note: Ensure the standard is completely dissolved and well mixed before use.
4. Standard Serial Dilution
Prepare seven tubes labeled No.1–7.
Add 300 μL of Standard Diluent to each.
Transfer 300 μL from No.0 to No.1, mix well, and continue the serial dilution up to No.6.
Tube No.7 serves as the blank (0 ng/mL).
Recommended standard concentrations: 10, 5, 2.5, 1.25, 0.625, 0.312, and 0.156 ng/mL.

Note: The reconstituted standard solution should be used immediately after preparation and must not bereused.
5. Biotinylated Antibody Preparation
Dilute 1:100 with Antibody Diluent 30 minutes before use. Prepare only the volume required and do notreuse.
6. HRP–Avidin Preparation
Dilute 1:100 with HRP–Avidin Diluent 30 minutes before use. Prepare only what is needed and do not reuse.
7. Color Reagent Preparation
Mix Color Reagent A and B in a 9:1 ratio. Prepare this mixture 30 minutes before use.

Storage

Store the kit at –20°C. For short-term storage (up to 2 weeks), keep at 2–8°C.
Shelf Life: 12 months from the production date.

Precision

Intra-Assay Precision: CV < 8%
Inter-Assay Precision: CV < 12%

Detection Range

0.156 – 10 ng/mL

Sensitivity

0.05 ng/mL

General Description

The Chicken Syndecan 4 (SDC4) ELISA Kit is designed for the quantitative determination of SDC4 concentrations in chicken samples such as serum, plasma, culture supernatants, or other biological fluids.
This kit employs a sandwich ELISA method using a pre-coated microplate with an anti-SDC4 monoclonalantibody and a biotinylated polyclonal detection antibody. The resulting immune complexes are detectedusing HRP–Avidin and TMB substrate, with color intensity measured at 450 nm being proportional to theSDC4 concentration.
For Research Use Only. Not for therapeutic or diagnostic applications!

Standard Curve