Anti-Smad1 Polyclonal Antibody (CABT-L2106) Knockout Validated

Rabbit Anti-Human Smad1 (His270~Ser465) Polyclonal Antibody for WB

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human, Bovine
Immunogen
Recombinant fragment corresponding to human SMAD1 (His270~Ser465)
Conjugate
Unconjugated

Applications


Application Notes
Recommended dilution: WB: 0.2-2 ug/ml; 1:250-2500
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Knockout Varification: Lane 1: Wild-type 293T cell lysate. Lane 2: Smad1 knockout 293T cell lysate. Predicted MW: 52kd, Observed MW: 60kd
Western Blot. Sample. Human 293T cell lysate.
Western Blot. Sample. Human Hela cell lysate.
Western Blot. Sample. Human HepG2 cell lysate.

Target


Alternative Names
BSP1; JV4-1; JV41; MADH1; MADR1; SMAD Family Member 1
Entrez Gene ID
UniProt ID

Product Background


Pathway
ALK1 signaling events; ALK2 signaling events; Angiogenesis; BMP receptor signaling; BMP signaling; BMP signalling and regulation; Delta-Notch Signaling Pathway; ErbB1 downstream signaling;

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


Schiemann, BJ; Neil, JR; et al. SPARC inhibits epithelial cell proliferation in part through stimulation of the transforming growth factor-beta-signaling system. MOLECULAR BIOLOGY OF THE CELL 14:3977-3988(2003).
Hassel, S; Schmitt, S; et al. Initiation of Smad-dependent and Smad-independent signaling via distinct BMP-receptor complexes. JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME 85A:44-51(2003).

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