Phospho-Smad1 (S463/S465)/Smad5 (S463/S465) ELISA Kit

Sample

cultured cells

Species Reactivity

Human; Mouse

Intended Use

Phospho-Smad1 (S463/S465)/Smad5 (S463/S465) ELISA Kit is an ELISA-based assay using fluorogenic substrates to measure phosphorylated Smad1/Smad5 in whole cells.

Contents of Kit

1. Phospho-Smad1 (S463/S465)/Smad5 (S463/S465) Antibody - 1 vial of lyophilized rabbit anti-phosphoSmad1 (S463/S465)/Smad5 (S463/S465) antibody.
2. Total GAPDH (Ms) Antibody - 1 vial of lyophilized mouse anti-GAPDH antibody.
3. HRP-conjugated anti-rabbit IgG - 110 μL of HRP-conjugated goat anti-rabbit IgG secondary antibody.
4. AP-conjugated anti-mouse IgG - 110 μL of AP-conjugated goat anti-mouse IgG secondary antibody.
5. Substrate F1 Concentrate - 50 μL of a sensitive and stable fluorogenic substrate for horseradish-peroxidase (HRP).
6. F1 Diluent - 10 mL of a solution for diluting the Substrate F1 Concentrate.
7. Substrate F2 - 10 mL of a sensitive and stable fluorogenic substrate for alkaline phosphatase (AP).
8. Blocking Buffer - 35 mL of 10% fetal bovine serum in buffer with preservatives.
9. Wash Buffer (5X) - 60 mL of a buffered surfactant with preservatives.
10. Microplate - 96-well cell culture clear-bottom black microplate and cover for use as a vessel in the assay.
11. Plate Sealers - 4 adhesive strips.

Storage

Store the unopened kit at 2-8°C. Do not use past kit expiration date. This kit contains sufficient materials to run ELISAs on one 96-well plate.