Anti-HSPA1A Polyclonal Antibody (CABT-L2029) Knockout Validated

Rabbit Anti-Human HSPA1A (Asp285~Asp641) Polyclonal Antibody for WB

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human, Mouse, Pig
Immunogen
Recombinant fragment corresponding to human HSPA1A (Asp285~Asp641)
Conjugate
Unconjugated

Applications


Application Notes
Recommended dilution: WB: 0.5-2 ug/ml
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Knockout Varification: Lane 1: Wild-type A549 cell lysate. Lane 2: HSPA1A knockout A549 cell lysate. Predicted MW: 64, 70kDa, Observed MW: 64kDa
Figure. Western Blot. Sample. Lane1: Porcine Liver Tissue; Lane2: Porcine Brain Tissue; Lane3: Mouse Liver Tissue; Lane4: Human A549 Cells .

Target


Alternative Names
HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70
Entrez Gene ID
UniProt ID

Product Background


Pathway
AUF1 (hnRNP D0) destabilizes mRNA; Antigen processing and presentation; Apoptosis Modulation by HSP70; Attenuation phase; Cellular response to heat stress; Cellular responses to stress; Direct p53 effectors; Disease;

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


Tulapurkar, ME; Ramarathnam, A; et al. Bacterial Lipopolysaccharide Augments Febrile-Range Hyperthermia-Induced Heat Shock Protein 70 Expression and Extracellular Release in Human THP1 Cells. PLOS ONE 10:-(2015).
Banerjee, D; Upadhyay, RC; et al. Seasonal variation in expression pattern of genes under HSP70. CELL STRESS & CHAPERONES 19:401-408(2014).

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