Anti-CD276 Polyclonal Antibody (CABT-L2113) Knockout Validated

Rabbit Anti-Human CD276 (Thr47~Ser219) Polyclonal Antibody for WB

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human
Immunogen
Recombinant fragment corresponding to human CD276 (Thr47~Ser219)
Conjugate
Unconjugated

Applications


Application Notes
Recommended dilution: WB: 0.5-2 ug/ml
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


ChoK1 transfected with human CD276 or Isotype control antibody, followed by CY5-conjugated Anti-rabbit IgG Secondary Antibody . Cells were fixed with 4% paraformaldehyde and permeabilized with 0. 1% Triton X-100.
Knockout Varification: Lane 1: Wild-type 293T cell lysate. Lane 2: CD276 knockout 293T cell lysate. Predicted MW: 57, 53, 33kd, Observed MW: 100kd
Figure. Western Blot. Sample. Lane1: Human 293T Cells; Lane2: Human PC-3 Cells; Lane3: Human MCF-7 Cells.

Target


Alternative Names
B7-H3; B7H3; CD276 Molecule; B7 homolog 3; Costimulatory molecule
Entrez Gene ID
UniProt ID

Product Background


Pathway
Cell adhesion molecules (CAMs);

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


Greaves, P; Gribben, JG; et al. The role of B7 family molecules in hematologic malignancy. BLOOD 121:734-744(2013).
Ng, RLX; Scott, NM; et al. Characterization of regulatory dendritic cells differentiated from the bone marrow of UV-irradiated mice. IMMUNOLOGY 140:399-412(2013).

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