Anti-C1q Autoantibodies ELISA Kit

Sample

serum, plasma

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

The CD anti-C1q Autoantibodies ELISA Kit is intended for the quantitative determination of anti-C1q autoantibodies in human serum or plasma.

Contents of Kit

1. Microtiter Plate, Precoated with human C1q
2. Plate Sealer
3. Wash Buffer Concentrate (10x)
4. Incubation Buffer
5. Calibrators A to D, Human serum matrix
6. Controls Low/High, Human serum matrix
7. Enzyme Label Anti-human IgG-HRP conjugate
8. TMB Substrate
9. Stop Solution

Storage

Unopened Reagents:
Store at 2-8°C. Do not use past kit expiration date.
Opened/Reconstituted Reagents:
1. Microtiter Plate: Reseal unused strips immediately to the foil pouch con-taining the desiccant packs and reseal along the entire edge of zip-seal. Store until expiration date at 2-8°C.
2. Wash Buffer: Store for up to 3 months at 2-8°C after dilution.
3. Calibrators, Controls, Incubation Buffer, Enzyme Label, TMB Substrate: Store at 2-8°C until expiration date.
4. Stop Solution: Stable at 18-28°C until expiration date marked on the vial.

Detection Limit

1.0 unit/ml

General Description

The C1q complex is potentially multivalent for attachment to the complement fixation sites of immunoglobulin. The sites are on the CH2 domain of IgG and, it is thought, on the CH4 domain of IgM. The appropriate peptide sequence of the complement fixing site might become exposed following complexing of the immunoglobulin, or the sites might always be available, but might require multiple attachment by C1q with critical geometry in order to achieve the necessary avidity.