Product Overview
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The UBE2H knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
Alternative Names
UBE2H; ubiquitin-conjugating enzyme E2H; ubiquitin-conjugating enzyme E2 H; UBCH2; ubiquitin-protein ligase H; ubiquitin carrier protein H; ubiquitin-conjugating enzyme E2-20K; Ubc8; E2-20K; N28148; AI181839; AI326965; AI462483; AW227540; 1500009C23Rik;
Application Notes
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Dilution
Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
Concentration
The protein concentration was determined with BCA assay.
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.
Citations
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Yi, JS; Park, JS; et al. MG53-induced IRS-1 ubiquitination negatively regulates skeletal myogenesis and insulin signalling. NATURE COMMUNICATIONS 4:-(2013).
Escalier, D; et al. New insights into the assembly of the periaxonemal structures in mammalian spermatozoa. BIOLOGY OF REPRODUCTION 69:373-378(2003).
COA
Certificate of Analysis
