Thromboxane B2 ELISA Kit

Sample

Urine, serum, tissue culture supernatants, and other sample matrices

Species Reactivity

Human

Detection Method

cELISA

Intended Use

The TXB2 ELISA Kit is a competitive assay that can be used for quantification of TXB2 in urine, serum, tissue culture supernatants, and other sample matrices. The assay has a range from 1.6-1,000 pg/ml and a sensitivity (80% B/B0) of approximately 5 pg/ml.

Contents of Kit

1. Thromboxane B2 ELISA Antiserum, 1 vial/100 dtn (96-well), 1 vial/500 dtn (480-well)
2. Thromboxane B2 AChE Tracer, 1 vial/100 dtn (96-well), 1 vial/500 dtn (480-well)
3. Thromboxane B2 ELISA Standard, 1 vial (96-well), 1 vial (480-well)
4. ELISA Buffer Concentrate (10X), 2 vials/10 ml (96-well), 4 vials/10 ml (480-well)
5. Wash Buffer Concentrate (400X), 1 vial/5 ml (96-well), 1 vial/12.5 ml (480-well)
6. Polysorbate 20, 1 vial/3 ml (96-well), 1 vial/3 ml (480-well)
7. Mouse Anti-Rabbit IgG Coated Plate, 1 plate (96-well), 5 plates (480-well)
8. 96-Well Cover Sheet, 1 cover (96-well), 5 covers (480-well)
9. Ellman's Reagent, 3 vials/100 dtn (96-well), 6 vials/250 dtn (480-well)
10. ELISA Tracer Dye, 1 vial (96-well), 1 vial (480-well)
11. ELISA Antiserum Dye, 1 vial (96-well), 1 vial (480-well)

Storage

This kit will perform as specified if stored as directed at -20°C and used before the expiration date indicated on the outside of the box.

Precision

The intra-and inter-assay CVs have been determined at multiple points on thestandard curve.These data are summarized below.

Table. Intra-assay variation *%CV represents the variation in concentration(not absorbance)as determinedusing a reference standard curve.

Detection Range

1.6-1,000 pg/ml

Sensitivity

Sensitivity (80% B/B0): 5 pg/ml.

General Description

Thromboxane A2 (TXA2) is produced from arachidonic acid by many cells and causes irreversible platelet aggregation and contraction of vascular and bronchial smooth muscle. Like most lipid mediators, TXA2 is not a circulating hormone. It is formed in response to local stimuli and exerts its effects within a short distance of its biosynthesis. TXA2 is rapidly hydrolyzed non-enzymatically to form TXB2, which is then quickly metabolized (t1/2 = 5-7 minutes) to urinary metabolites for clearance by the kidneys. Because of the transient nature of this compound it is difficult to accurately measure circulating levels in whole animal experimental models. In fact, it has been shown that plasma and urine levels of TXB2 are primarily due to ex vivo platelet activation and intra-renal production, respectively. Therefore, measurement of TXB2 metabolites, such as 11-dehydro TXB2, in urine and plasma may give more accurate estimates of in vivo TXA2 production. TXB2 measurement is better suited for samples that are unlikely to undergo extensive metabolism such as perfusates, lavage samples, tissue/cell culture, etc.

Standard Curve

The standard curve presented here is an example of the data typically producedwith this kit; however,your results will not be identical to these.You must run a new standard curve. Do not use the data below to determine the values of yoursamples. Your results could differ substantially.