11-dehydro Thromboxane B₂ ELISA Kit

Sample

serum, plasma

Detection Method

cELISA

Intended Use

The 11-dehydro TXB₂ ELISA Kit is a competitive assay that can be used for the quantification of 11-dehydro TXB₂ in plasma, serum, urine, and other sample matrices. The assay has a range of 15.6-2,000 pg/ml, with a midpoint(50% B/B0) of 80-160 pg/ml, and a sensitivity(80% B/B0) of approximately 34 pg/ml.

Contents of Kit

1. 11-dehydro Thromboxane B₂ AChE Tracer
2. 11-dehydro Thromboxane B₂ Monoclonal Antibody
3. 11-dehydro Thromboxane B₂ ELISA Standard
4. 11-dehydro Thromboxane B₂ Assay Buffer Concentrate(10X)
5. Wash Buffer Concentrate (400X)
6. Polysorbate 20
7. Goat Anti-Mouse lgG-Coated Plate
8. Ellman's Reagent
9. ELISA Tracer Dye
10. ELISA Antiserum Dye
11. 96-Well Cover Sheet

Performance Characteristics

Assay Range = 15.6-2,000 pg/ml
Sensitivity (defined as 80% B/Bo) = 34 pg/ml
Mid-point (defined as 50% B/Bo) = 120 pg/ml
LLOD = 16 pg/ml
The sensitivity and mid-point were derived from the standard curve shown above. The standard was diluted with 11-dehydro TXB₂ Assay Buffer (1X).

Standard Curve