Tobacco Mosaic Virus (TMV) ELISA Kit

Name: Tobacco Mosaic Virus (TMV) ELISA Kit
SKU: DEIAPV3
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Host plans

Species Reactivity

Plant

Detection Method

sELISA

Intended Use

The test can be used to detect TMV in infected host plants include vegetable, fruit, and ornamentals.

Contents of Kit

500 wells
1. Coating antibody: 0.250 ml, 4°C
2. Detecting conjugate, alkaline phosphatase: 0.250 ml, 4°C
3. Negative control: 1 vial
4. Positive control: 1 vial
5. Coating Buffer 10×: 6 mL
6. SB1 Buffer: 11.6 g
7. PBST Buffer: 28.7 g
8. ECB1 Buffer 10×: 6 mL
9. PNP Buffer 5×: 10 mL
10. PNP tablet: 10 tablets
11. Tween-20: 7 g
12. 96-well ELISA plates: 5
13. Instruction: 1
1000 wells
1. Coating antibody: 0.50 ml, 4°C
2. Detecting conjugate, alkaline phosphatase: 0.50 ml, 4°C
3. Negative control: 1 vial
4. Positive control: 1 vial
5. Coating Buffer 10×: 12 mL
6. SB1 Buffer: 23.2 g
7. PBST Buffer: 57.23 g
8. ECB1 Buffer 10×: 12 mL
9. PNP Buffer 5×: 20 mL
10. PNP tablet: 20 tablets
11. Tween-20: 14 g
12. 96-well ELISA plates: 10, Room temperature
13. Instruction: 1

Storage

Store all buffers at 2-4°C, except for the PNP tablets at -20°C under dark.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Efficacy of the subthreshold micropulse yellow wavelength laser photostimulation in the treatment of chronic central serous chorioretinopathy

INTERNATIONAL JOURNAL OF OPHTHALMOLOGY

Authors: Isik, Mehmed Ugur; Degirmenci, Mehmet Fatih Kagan; Saglik, Ayhan

Abstract

AIM: To evaluate the efficacy and safety of subthreshold micropulse yellow laser (SMYL) in the treatment of chronic central serous chorioretinopathy (CCSC). METHODS: The medical records of 58 eyes of 58 patients with CCSC were reviewed. A 577-nm SMYL system was used for the treatment. Fundus fluorescein angiography was used as the primary method of identifying CCSC, and resolution of subretinal fluid (SRF) evaluated by optical coherence tomography (OCT) and fundus autofluorescence. Central macular thickness (CMT), central macular volume (CMV), total macular volume (TMV), subfoveal choroidal thickness (SFCT), subretinal fluid height (SRFH), and subfoveal fluid basement diameter values were measured by spectral domain-OCT (SD-OCT) for all eyes. RESULTS: The mean age of the patients was 42.4 +/- 9.9 (range: 20-72)y. The mean follow-up was 11.4 +/- 8.5 (range: 6-37)mo. Median BCVA at at the final follow up after treatment was statistically significant from the baseline. Complete SRF resolution was 12.1% of the eyes in the 1st month, 67.2% of the eyes in the 3rd month and 67.2% of the eyes in the last follow up. The initial median CMT, CMV, TMV, and SFCT values before treatment was significantly higher than 3rd month visit values (P<0.001). In the multivariate analysis performed, age and disease duration were found to be a risk factor for persistent SRF (P=0.017, P=0.016, respectively). CONCLUSION: SMYL treatment provides a significant anatomical and functional improvement and is effective in eliminating SRF in eyes with CCSC.

Anti-TMV activity and functional mechanisms of two sesquiterpenoids isolated from Tithonia diversifolia

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY

Authors: Zhao, Lihua; Dong, Jiahong; Hu, Zhonghui; Li, Shunlin; Su, Xiaoxia; Zhang, Jie; Yin, Yueyan; Xu, Tao; Zhang, Zhongkai; Chen, Hairu

Abstract

Unlike chemical pesticides, antiviral plants are biodegradable, replenishable and safe. In this study, 14 sesquiterpene compounds from Tithonia diversifolia were tested for their activities against Tobacco mosaic virus (TMV) using the half-leaf method. Tagitinin C (Ses-2) and 1 beta-methoxydiversifolin-3-0-methyl ether (Ses-5) were found to have in vivo curative activities of 62.86% and 60.27% respectively, at concentrations of 100 mu g/mL, respectively. In contrast, the in vivo curative inhibition rate of control agent ningnanmycin was 52.48%. Indirect enzyme-linked immunosorbent assay (ID-ELISA) also verified Ses-2 and Ses-5 had higher inhibition activities than the control agent ningnanmycin. Additionally, qRT-PCR showed that both Ses-2 and Ses-5 can partly inhibit the expression of CP and RdRp, two genes that play key roles in TMV infection. When TMV started to systemically spread, Ses-2 inhibited CP expression while Ses-5 inhibited RdRp expression. These results suggest that the two bio-agents have anti-TMV activities and may be used as bio-pesticides to control the plant virus. 2017 The Authors. Published by Elsevier Inc.