Enhanced foreign protein accumulation in Nicotiana benthamiana leaves co-infiltrated with a TMV vector and plant cell cycle regulator genes
Authors: Kopertekh, Lilya; Schiemann, Joachim
In this short communication, we report that the cell cycle checkpoint genes At-CycD2 and At-CDC27a from Arabidopsis thaliana enhance the transient heterologous protein expression in Nicotiana benthamiana. We selected a well-studied and widely used virus expression vector based on TMV for the delivery of recombinant proteins into the host plant. Co-infiltration of TMV-gfp and binary expression vectors carrying the At-CycD2 and At-CDC27a genes, respectively, resulted in enhanced GFP fluorescence in agroinoculated leaves. These findings corresponded with the observation of (1) higher mRNA levels for TMV and gfp and (2) increased GFP protein accumulation. Furthermore, by co-delivery of the TMV-scFv-TM43-E10 and At-CycD2/At-CDC27a expressing constructs we observed an enhanced amount of the scFv-TM43-E10 antibody fragment compared to the delivery of the TMV-scFv-TM43-E10 alone. We anticipate that this finding might be adapted for enhancing foreign protein production in N. benthamiana as the host plant.
Derivatives of Isonicotinic Acid as New Efficient Systemic Acquired Resistance (SAR) Inducers
Authors: Czerwoniec, Patrycja; Lewandowski, Piotr; Smiglak, Marcin
Systemic acquired resistance (SAR) is one of the most promising ways to support plants in fight against viruses, bacteria and fungi. This phenomenon is activated by pathogen attack or artificially, by using resistance inducer (elicitor) which imitates the plant-pathogen interaction. Both, biological or chemical factors interact with plants and stimulate their immune system against infections before first symptoms of diseases occurs. SAR inducing properties of elicitors could be potentially utilized to develop a new plant protection strategies. This paper presents synthesis, phytotoxicity and SAR induction efficacy of four newly obtained amide derivatives of 2,6-dichloroisonicotinic acid and four known amide derivatives of isonicotinic acid. Induction of plant resistance tests were performed on tobaccoNicotiana tabacumvar.Xanthiinfected byTobacco mosaic virus(TMV). Six of the eight presented substances indicate resistance inducing properties, which were expressed by decrease of necrotic area on leaves caused by viral infection by 44 to 92 % in comparison to the control plants. Moreover, obtained results show that presented compounds exhibited better plant resistance inducing properties than salicylic acid, oxalic acid and beta-aminobutyric acid used as reference.