Spectinomycin ELISA Kit

Sample

Tissue(chicken,chicken liver, pork, swine liver, fish, shrimp)

Species Reactivity

N/A

Detection Method

cELISA

Intended Use

This kit can be used in quantitative and qualitative analysis of spectinomycin residue in tissue(chicken, chicken liver, pork, swine liver, fish, shrimp) etc.

Contents of Kit

1.Microtiter plate with 96 wells coated with antigen
2.Standard solutions(6 bottles,1ml/bottle)
0 ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb, 16.2ppb
3.Spiking standard solution:(1ml/bottle) 1 ppm
4.Enzyme conjugate 7ml…………………..…red cap
5.Antibody solution 7ml.………..……….…green cap
6.Solution A 7ml ……………………….....…white cap
7.Solution B 7ml …………………….….…..…red cap
8.Stop solution 7ml……………………...…yellow cap
9.20×Concentrated wash solution 40ml… transparent cap
10.2×Concentrated extraction solution 50ml… transparent cap

Storage

Storage condition: 2-8°C.
Storage period: 12 months.

Precision

Variation coefficient of the ELISA kit is less than 10%.
Accuracy
milk……………………….…………..90±20%

Detection Limit

3ppb

Sensitivity

0.2ppb

General Description

Spectinomycin is an aminocyclitol antibiotic, which is a new special antibiotic for gonorrhea therapy. It can stop protein synthesis of cytoderm of bacteria and prevent synthesized protein release, so it has strong bacteriostasis. This kit is a new product based on ELISA technology, which is fast, easy, accurate and sensitive compared with common instrumental analysis and only needs 45min in one run, so it can considerably minimize operation error and work intensity.

Standard Curve

1.To draw a standard curve: Take the absorbance value of standards as y-axis, semi logarithmic of the concentration of the spectinomycin standards solution (ppb) as x-axis.
2.The spectinomycin concentration of each sample (ppb), which can be read from the calibration curve, is multiplied by the corresponding Dilution factor of each sample followed, and the actual concentration of sample is obtained. Notice: Special software has been developed for result calculation, which can be provided on request.