Intended Use
The STAT3 (Phospho-Tyr705) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor STAT3 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated STAT3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on STAT3 phosphorylation.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate. 2 Plates
2. 10× TBS, 24 ml (10×), Stability after first use: 1 month at 4°C
3. Quenching Buffer, 24 ml (1×), Stability after first use: 1 month at 4°C
4. Blocking Buffer, 50 ml (1×), Stability after first use: 1 month at 4°C
5. 15× Wash Buffer, 50 ml (15×), Stability after first use: 1 month at 4°C
6. 100× Anti-STAT3 (Phospho-Tyr705) Antibody, 60 μl (100×), Stability after first use: 1 month at 4°C
7. 100× Anti-STAT3 Antibody, 60 μl (100×), Stability after first use: 1 month at 4°C
8. 100× Anti-GAPDH Antibody, 60 μl (100×), Stability after first use: 1 month at 4°C
9. HRP-Conjugated Anti-Rabbit IgG Antibody, 12 ml (1×), Stability after first use: 1 month at 4°C
10. HRP-Conjugated Anti-Mouse IgG Antibody, 12 ml (1×), Stability after first use: 1 month at 4°C
11. Primary Antibody Diluent, 12 ml (1×), Stability after first use: 1 month at 4°C
12. Ready to Use Substrate, 12 ml (1×), Stability after first use: 1 month at 4°C
13. Stop Solution, 12 ml (1×), Stability after first use: 1 month at 4°C
14. Crystal Violet Solution, 12 ml (1×), Stability after first use: 1 month at 4°C
15. SDS Solution, 24 ml (1×), Stability after first use: 1 month at 4°C
16. Adhesive Plate Seals, 2 Seals.
COA
Publication 
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