Product Overview
Escherichia coli derived. Streptomyces avidinii streptavidin (protein accession #. CAA00084, Asp2-Gln160) fused with a C-terminal Renilla reniformis luciferase. Containing an N-terminal 6× histidine tag.
Purity
> 95%, determined by SDS-PAGE under reducing conditions and visualized by Commassie blue staining (see the image below).
Format
Liquid, clear solution. Supplied as a 0. 2 μm filtered solution in PBS (pH 7. 4).
Storage
2-8°C short term, -20°C long term
Introduction
Streptavidin is atetrameric protein purified from Streptomyces sp. that binds very tightly tothe vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognitionof biotin and biotinylated molecules has made streptavidin one of the mostimportant components in diagnostics and laboratory kits.
Keywords
Streptavidin; SA V1; SA V2; Streptavidin V1; Streptavidin V2; Luciferase; ec 1.13.12.7; Firefly; Luciferin 4 monooxygenase; Streptavidin-Luciferase
Citations
Publication ()
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COA
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