Mouse CSF2 peptide

Proper embryonic development depends upon regulatory signals from the reproductive tract called embryokines. This review uses the cow as a model to understand the developmental processes controlled by embryokines. The focus is on 3 embryokines that have been shown to increase competence of embryos to survive after transfer to recipients: CSF2, IGF1, and DKK1. Together, these molecules regulate key events required for development of the embryo to the blastocyst stage including proliferation (enhanced by IGF1), development of the inner cell mass (regulated by CSF2), control of differentiation (modified by DKK1), and inhibition of apoptosis and stress-mediated developmental arrest (IGF1 and CSF2).

A cDNA library was constructed from poly(A)(+)RNA extracted from pollinated fruit of 'PMR-142' cucumber (Cucumis sativus L.), Subtraction hybridization was made between the cDNAs and poly(A)(+)RNA from unpollinated fruit to isolate cDNA clones that corresponded to the genes preferentially expressed in the pollinated fruit, We isolated three cDNAs, which were 756, 826, and 998 nucleotides long and designated Csf1, Csf2, and Csf3, respectively. When fruit growth was triggered by pollination, auxin treatment and natural parthenocarpy, Csf2 was always expressed. Time course of expression of the Csf2 gene was nearly parallel to that of the fruit growth. Nucleotide sequences of the Csf cDNAs were fully determined. Homology of the deduced amino acid sequence for Csf1 showed 75 % identity with a pea extensin. Only 37%, 33%, and 26% homology was found between Csf2 and hell pepper CaSn-2, tobacco FB7-4, and opium poppy gMLP15, respectively. The Csf3 sequence showed 68% identity with the large subunit of 60S ribosomal protein L3 of Arabidopsis thaliana.