Batch dependent - please inquire should you have specific requirements.
Buffer
20mM PB, 150mM NaCl, pH 7.4
Preservative
None
Storage
Store at -20°C to -80°C. Avoid multiple freeze/thaw cycles.
Introduction
Mycobacterium tuberculosis is a species of pathogenic bacteria in the family Mycobacteriaceae and the causative agent of tuberculosis.First discovered in 1882 by Robert Koch, M. tuberculosis has an unusual, waxy coating on its cell surface primarily due to the presence of mycolic acid. This coating makes the cells impervious to Gram staining, and as a result, M. tuberculosis can appear either Gram-negative or Gram-positive.
Antigen Description
M. Tuberculosis (H37Rv) CFP10 protein (a.a 1-104) was expressed in E.coli and fused a 6x His-tag at the C-terminus
Keywords
M. Tuberculosis CFP-10 Protein; M. Tuberculosis CFP-10; M. Tuberculosis; Mycobacterium tuberculosis; CFP-10
Citations
Publication ()
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Background
Mycobacterium tuberculosis (M. tuberculosis) is a specialized aerobic rod-shaped bacterium that causes tuberculosis (TB), is slow-growing, highly contagious, and is subject to a host immune response upon contact with host cells, leading to latent TB infection or complete clearance of the bacteria. M. tuberculosis has several strategies to evade the immune system. Different antigens are currently being used to develop various TB vaccines. Among them, subunit vaccines are one of the most commonly used vaccines, and the current selection regarding vaccine antigens focuses on the major marker antigens of M. tuberculosis, such as PPE44, HSPX, ESAT-6 and CFP-10.
The M. tuberculosis H37Rv genome consists of 36 regions of difference (RDs), of which the RD1 motif plays a key role in the virulence of this bacterium and encodes the bacterial protective or virulence antigens, including the genes that encode ESAT-6, CFP-10. These two genes form a heterodimeric complex in a 1:1 ratio. Culture filtrate protein-10 (CFP-10) can modulate the host immune response by forming a complex with ESAT-6, which can enter the endoplasmic reticulum and dissociate Beta-2 microglobulin (β2M) from the pMHC-I complex, thereby inhibiting the expression of the MHC-I-β2M complex on the cell surface and leading to the down-regulation of class I-mediated antigen presentation. CFP-10 activates and aggregates cytotoxic T lymphocytes and expands granulomatous tissue in humans and mice infected with M. tuberculosis. The secreted proteins of ESAT-6 and CFP-10 are produced only in the early stages of tuberculosis infection, and deletion of the genes encoding the secreted proteins of the pathogenic strains has been found to reduce the virulence of the bacteria.
Figure 1. The location of esat-6 and cfp-10 genes on RD1 locus (Source: Valizadeh A, et al. 2022)
ESAT-6 and CFP-10 are also highly immunogenic secreted proteins used in TB diagnostics and can be used in interferon gamma release assays (IGRAs) and skin tests. Only ESAT-6 and CFP-10 from M. tuberculosis can be used to stimulate lymphocytes when IGRAs are assayed, these antigens are absent in Mycobacterium bovis and BCG. Two skin tests using only two specific MT antigens (ESAT-6 and CFP-10) rather than tuberculin solution have been developed for specific M. tuberculosis antigen skin tests with similar specificity to the IGRA.
Alternative Names
Mycobacterium tuberculosis CFP-10
References
1. Valizadeh A, et al. Evaluating the Performance of PPE44, HSPX, ESAT-6 and CFP-10 Factors in Tuberculosis Subunit Vaccines. Curr Microbiol. 2022 Jul 19;79(9):260.
2. Zellweger JP, et al. The diagnosis of latent tuberculosis infection (LTBI): currently available tests, future developments, and perspectives to eliminate tuberculosis (TB). Med Lav. 2020 Jun 26;111(3):170-183.
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References
Surface plasmon resonance biosensor chips integrated with MoS2-MoO3 hybrid microflowers for rapid CFP-10 tuberculosis detection
J Mater Chem B
Authors: Wulandari C, Septiani NLW, Gumilar G, Nuruddin A, Nugraha, Iqbal M, Wasisto HS, Yuliarto B.
This study reports on the modification of surface plasmon resonance (SPR) chips with molybdenum disulfide-molybdenum trioxide (MoS2-MoO3) microflowers to detect the tuberculosis (TB) markers of CFP-10. The MoS2-MoO3 microflowers were prepared by hydrothermal methods with variations in the pH and amount of trisodium citrate (Na3Ct), which were projected to influence the shape and size of microflower particles. The analysis shows that optimum MoS2-MoO3 hybrid microflowers were obtained at neutral pH using 0.5 g Na3Ct. The modified SPR biosensor exhibits a ten times higher response than the bare Au. Moreover, increasing MoS2-MoO3 thickness results in a higher detection response, sensitivity, and a smaller limit of detection (LOD). Using the optimized material composition, the Au/MoS2-MoO3-integrated SPR sensor can demonstrate sensitivity and LOD of 1.005 and 3.45 ng mL-1, respectively. This biosensor also has good selectivity, stability, and reproducibility based on cross-sensitivity characterization with other analytes and repeated measurements on several chips with different storing times and fabrication batch. Therefore, this proposed SPR biosensor possesses high potential to be further developed and applied as a detection technology for CFP-10 in monitoring and diagnosing TB.
Detection of mycobacterial CFP-10 (Rv3874) protein in tuberculosis patients by gold nanoparticle-based real-time immuno-PCR
Future Microbiol
Authors: Dahiya B, Sharma S, Khan A, Kamra E, Mor P, Sheoran A, Sreenivas V, Varma-Basil M, Gupta KB, Gupta MC, Chaudhary D, Mehta PK.
Aim: Timely and reliable diagnostic test for tuberculosis (TB) is immediately required. Attempts were made to improve the technology and diagnostic potential of real-time immuno-PCR (RT-I-PCR). Methods: We designed gold nanoparticle (GNP)-based RT-I-PCR (GNP-RT-I-PCR) assay for the detection of Mycobacterium tuberculosis CFP-10 (Rv3874) protein in clinical samples of TB patients. Results: A wide quantitative detection range of CFP-10 was found to be 0.5-5 × 104 pg/ml in bodily fluids of TB patients, which can evaluate the progression of disease. Moreover, sensitivities of 83.7 and 76.2% were observed in pulmonary (n = 49) and extrapulmonary TB (n = 42) patients, respectively, with specificities of 93.5-93.8% (n = 63). Conclusion: Conjugation of detection antibodies and oligonucleotides to functionalized GNPs of GNP-RT-I-PCR is relatively easier, compared with streptavidin-biotin/succinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate system employed in RT-I-PCR. Our assay also showed better diagnostic performance than RT-I-PCR, which may provide a viable platform for the development of an efficient TB diagnostic test.
COA
Publication 
Figure 1. The location of esat-6 and cfp-10 genes on RD1 locus
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