Recombinant HAV VP3 (a.a. 248-491) [His]

Product Overview

C-terminal 6xHis tagged VP3 region of HAV capsid protein (a.a. 248-491)

Nature

Recombinant

Tag/Conjugate

His

Purity

> 95%, based on SDS PAGE

Format

Each vial contains 100 μg of lyophilized protein in PBS with 8M Urea.

Preservative

None

Storage

2-8°C short term, -20°C long term

Introduction

Hepatitis A virus (HAV) is the sole member of the Hepatovirus genus within the family Picornaviridae. The capsid of HAV encloses a single-stranded RNA genome of about 7.5 kb which is translated into a single polyprotein. The virion proteins VP1 to VP4 and the nonstructural proteins are generated from the polyprotein by a cascade of proteolytic cleavages. Only one protease, viral protease 3C, has been implicated in the nine protein scissions. Processing of the capsid protein precursor region generates a unique intermediate, PX (VP1-2A), which accumulates in infected cells and is assumed to serve as precursor to VP1 found in virions, although the details of this reaction have not been determined. Capsid proteins VP1, VP2, and VP3 form a closed capsid enclosing the viral positive strand RNA genome. VP1 is a major viral antigen.

Keywords

Picornaviridae; Hepatovirus; Hepatitis A virus; Hepatitis A virus VP3 protein; HAV VP3; infectious hepatitis and epidemical virus