HAV VP3 [GST]

Product Overview

Recombinant Hepatitis A Virus antigen (38.6 kDa), contains VP3 immunodominant region and a GST fusion partner, was expressed in Ecoli, and puried in vitro using Metal affinity chromatography techniques.

Nature

Recombinant

Tag/Conjugate

GST

Molecular Weight

38.6 kDa

Purity

> 90% pure (10% PAGE coomassie staining). Inclusion Bodies

Format

Purified, Liquid

Concentration

1 mg/ml

Buffer

10mM Carbonate-bicarbonate buffer, pH 9.6, 0.1% SDS containing 50% glycerol

Preservative

None

Storage

2-8°C short term, -20°C long term

Introduction

Hepatitis A virus (HAV) is the sole member of the Hepatovirus genus within the family Picornaviridae. The capsid of HAV encloses a single-stranded RNA genome of about 7.5 kb which is translated into a single polyprotein. The virion proteins VP1 to VP4 and the nonstructural proteins are generated from the polyprotein by a cascade of proteolytic cleavages. Only one protease, viral protease 3C, has been implicated in the nine protein scissions. Processing of the capsid protein precursor region generates a unique intermediate, PX (VP1-2A), which accumulates in infected cells and is assumed to serve as precursor to VP1 found in virions, although the details of this reaction have not been determined. Capsid proteins VP1, VP2, and VP3 form a closed capsid enclosing the viral positive strand RNA genome. VP1 is a major viral antigen.

Keywords

HAV; HAV VP1-P2A; Hepatitis A Virus VP1-P2A; Picornaviridae; Hepatovirus