Bacillus Thermolysin (DAG2606)

Bacillus Thermolysin, recombinant protein from Bacillus

Product Overview
Recombinant Bacterial Thermolysin antigen was expressed in Bacillus thermoproteolyticus rokko.
Nature
Recombinant
Tag/Conjugate
Unconjugated
Procedure
None
Purity
> 95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Preservative
None
Storage
2-8°C short term, -20°C long term
Introduction
Like all bacterial extracellular proteases thermolysin is first synthesised by the bacterium as a pre-proenzyme. Thermolysin is synthesized as a pre-proenzyme consisting of a signal peptide 28 amino acids long, a pro-peptide 204 amino acids long and the mature enzyme itself 316 amino acids in length. The signal peptide acts as a signal for translocation[disambiguation needed] of pre-prothermolysin to the bacterial cytoplasmic membrane. In the periplasm pre-prothermolysin is then processed into prothermolysin by a signal peptidase. The prosequence then acts as a molecular chaperone and leads to autocleavage of the peptide bond linking pro and mature sequences. The mature protein is then secreted into the extracellular medium
Antigen Description
Thermolysin EC 3.4.24.27 is a thermostable neutral metalloproteinase enzyme produced by the Gram-positive bacteria Bacillus thermoproteolyticus. It requires one zinc ion for enzyme activity and four calcium ions for structural stability. Thermolysin specifically catalyzes the hydrolysis of peptide bonds containing hydrophobic amino acids. However thermolysin is also widely used for peptide bond formation through the reverse reaction of hydrolysis. Thermolysin is the most stable member of a family of metalloproteinases produced by various Bacillus species. These enzymes are also termed 'neutral' proteinases or thermolysin -like proteinases (TLPs).
Keywords
Bacillus thermoproteolyticus neutral proteinase; Thermolysin; B. thermoproteolyticus Thermolysin protein

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