Recombinant Bacillus Thermolysin

Product Overview

Recombinant Bacterial Thermolysin antigen was expressed in Bacillus thermoproteolyticus rokko.

Nature

Recombinant

Tag/Conjugate

Unconjugated

Purity

> 95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Preservative

None

Storage

2-8°C short term, -20°C long term

Introduction

Like all bacterial extracellular proteases thermolysin is first synthesised by the bacterium as a pre-proenzyme. Thermolysin is synthesized as a pre-proenzyme consisting of a signal peptide 28 amino acids long, a pro-peptide 204 amino acids long and the mature enzyme itself 316 amino acids in length. The signal peptide acts as a signal for translocation[disambiguation needed] of pre-prothermolysin to the bacterial cytoplasmic membrane. In the periplasm pre-prothermolysin is then processed into prothermolysin by a signal peptidase. The prosequence then acts as a molecular chaperone and leads to autocleavage of the peptide bond linking pro and mature sequences. The mature protein is then secreted into the extracellular medium

Keywords

Bacillus thermoproteolyticus neutral proteinase; Thermolysin; B. thermoproteolyticus Thermolysin protein