Tag/Conjugate
Unonjugated
Molecular Weight
34.6 kDa by amino acid sequence
Alternative Names
Thermolysin; Geobacillus stearothermophilus; Bacillus Thermolysin
Format
Lyophilized powder containing calcium and sodium acetate buffer salts
Concentration
30-350 units/mg protein (E1%/280)
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Buffer
Lyophilized powder containing calcium and sodium acetate buffer salts
Introduction
Thermolysin (EC 3.4.24.27) is a highly thermostable, zinc-dependent metalloendopeptidase produced by the Gram-positive bacterium Bacillus thermoproteolyticus. It is widely used in biochemical research and biotechnology to selectively cleave peptide bonds at the N-terminus of hydrophobic residues (Leu, Phe, Val, Ile, Ala, Met).
Keywords
Thermolysin; Geobacillus stearothermophilus; Bacillus Thermolysin
Citations
Publication ()
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COA
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