Prostaglandin E2 ELISA Kit

Sample

biological samples

Detection Method

Competitive ELISA

Intended Use

This competitive ELISA kit is for determination of PGE2 (Prostaglandin E2) levels in biological samples.
Each kit can be used for triplicate analyses of up to 24 samples contains using a 96 well plate format, and contains a vial of PGE2 standard, a vial of PGE2-conjugated horseradish peroxidase (HRP), and buffers for sample and HRP dilutions, and plate washing.

Contents of Kit

1. PGE2 ELISA Plate: Solid 96-well plate coated with anti-PGE2 antibody in each well
2. PGE2 Standard (2 μL): Stock standard at a concentration of 1 mg/mL
3. PGE2-HRP Conjugates (12 μL): 1000 X concentrated solution
4. Sample Dilution Buffer (25 mL): 10 X solution of Tris-buffered saline with preservatives
5. HRP Buffer (15 mL): 1 X solution of Tris-buffered saline with preservatives
6. Wash Buffer Solution (25 mL): 10 X solution of Tris-buffered saline with detergents and preservatives
7. TMB Substrate (24 mL): A solution of TMB (tetra methyl benzadine)

Storage

This kit will obtain optimal results if all of the components are stored at the proper temperature prior to use. Items should be stored at the designated temperatures upon receipt of this kit.

All components are stored below -20°C and should not be re-frozen and thawed more than necessary.

Standard Curve