Prostaglandin E2 ELISA Kit

Name: Prostaglandin E2 ELISA Kit
SKU: DEIA4977
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Urine, plasma, tissue culture supernatants, and other sample matrices

Species Reactivity

Human

Detection Method

cELISA

Intended Use

PGE2 ELISA Kit is a competitive assay that can be used for quantification of PGE2 in urine, plasma, tissue culture supernatants, and other sample matrices. The assay has a range from 7.8-1,000 pg/ml, a sensitivity (80% B/B0) of approximately 13 pg/ml, and a lower limit of detection (LLOD) of 11 pg/ml.

Contents of Kit

1. Prostaglandin E2 Monoclonal Antibody, 1 vial/100 dtn (96 wells), 1 vial/500 dtn(480 wells)
2. Prostaglandin E2 AChE Tracer, 1 vial/100 dtn (96 wells), 1 vial/500 dtn(480 wells)
3. Prostaglandin E2 ELISA Standard, 1 vial (96 wells), 1 vial(480 wells)
4. ELISA Buffer Concentrate (10×), 2 vials/10 ml (96 wells), 4 vials/10 ml(480 wells)
5. Wash Buffer Concentrate (400×), 1 vial/5 ml (96 wells), 1 vial/12.5 ml(480 wells)
6. Polysorbate 20, 1 vial/3 ml (96 wells), 1 vial/3 ml(480 wells)
7. Goat Anti-Mouse IgG Coated Plate, 1 plate (96 wells), 5 plates(480 wells)
8. 96-Well Cover Sheet, 1 cover (96 wells), 5 covers(480 wells)
9. Ellman' s Reagent, 3 vials/100 dtn (96 wells), 6 vials/250 dtn(480 wells)
10. ELISA Tracer Dye, 1 vial (96 wells), 1 vial(480 wells)
11. ELISA Antiserum Dye, 1 vial (96 wells), 1 vial(480 wells)

Storage

This kit will perform as specified if stored as directed at -20°C and used beforethe expiration date indicated on the outside of the box.

Precision

The intra-and inter-assay CVs have been determined at multiple points on thestandard curve.

Intra-and inter-assay variation
*%CV represents the variation in concentration(not absorbance)as determinedusing a reference standard curve.

Detection Range

7.8-1000 pg/ml

Detection Limit

Lower limit of detection (LLOD): 11 pg/ml

Sensitivity

Sensitivity (80% B/B₀): 13 pg/ml

General Description

Prostaglandin E2 (PGE2) is an eicosanoid formed from arachidonic acid by COX enzymes and PGE2 synthase (PGES) via PGG2 and PGH2 intermediates. It can be produced de novo in all cells following cell activation and the release of arachidonic acid from plasma membrane phospholipids or when exogenous free arachidonate is available. It acts in an autocrine or paracrine fashion to bind to its receptors, EP1, EP2, EP3, and EP4, which have differing tissue distribution and cellular localizations, to initiate signaling through various pathways, including the protein kinase A (PKA), β-catenin, EGF, MAPK, NF-κB, and PI3K/Akt pathways. Due to this, PGE2 is involved in a wide variety of biological processes, including inflammation, fertility and parturition, gastric mucosal integrity and motility, and immune modulation in and outside of cancer.

Standard Curve

Sample Data
The standard curve presented here is an example of the data typically produced with this kit;however, your results will not be identical to these. You must run anew standard curve.Do not use the data below to determine the values of your samples. Your results could differ substantially.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Walking metals: catalytic difunctionalization of alkenes at nonclassical sites

CHEMICAL SCIENCE

Authors: Dhungana, Roshan K.; Sapkota, Rishi R.; Niroula, Doleshwar; Giri, Ramesh

Abstract

Migration of metals along a carbon chain is triggered by two of the most common organometallic elementary steps - beta-hydride (beta-H) elimination and alkene hydrometallation. This process heralds a new future for creating bonds at carbon sites that fall outside the tenets of the conventional wisdom for reactivity and bond formation, and provides an opportunity to leverage beta-H elimination to advance the very reaction of alkene difunctionalization it is intrinsically predestined to disrupt. Almost four decades since its genesis, the early adventure for alkene difunctionalization by metal migration was sporadic, and its later development went on a hiatus primarily due to original impetus on arresting beta-H elimination for vicinal alkene difunctionalization. With the recent surge on alkene difunctionalization, efforts have been gradually shifting to harnessing the process of beta-H elimination to difunctionalize alkenes at sites other than the classical vicinal carbons, termed henceforth nonclassical reaction sites for pedagogical simplicity. In this review article, we extricate and examine the origin and the development of such reactions over the years. This review covers a wide range of reactions for the difunctionalization of alkenes at geminal (1,1), allylic (1,3) and remote (1,n) carbon sites with a variety of coupling partners. These reactions have enabled engineering of complex molecular frameworks with the generation of new carbon-carbon (C-C)/C-C, C-C/C-heteroatom (halogens, O, N, B) and C-B/C-B bonds. The development of these unique transformations is also presented with mechanistic hypotheses and experimental evidences put forward by researchers. Judged by the number of reports emerging recently, it is now strikingly evident that the field of alkene difunctionalization by metal migration has begun to gain momentum, which holds a great future prospect to develop into a synthetic method of enormous potential.

Behavioural responses of female lake troutSalvelinus namaycushto male chemical stimuli and prostaglandinF(2 alpha)

JOURNAL OF FISH BIOLOGY

Authors: Buchinger, Tyler J.; Li, Weiming; Johnson, Nicholas S.

Abstract

Male olfactory cues may guide aggregation on spawning reefs, mate evaluation and synchronized gamete release in lake troutSalvelinus namaycush, but a lack of information on the source and identity of the cues precludes direct tests of their function. Using a two-channel flume, we found ovulated female lake trout increased time spent in the channel treated with spermiated male-conditioned water, urine and bile but not synthesized prostaglandin F-2 alpha. We suggest future efforts to characterize male olfactory cues focus on urine and postulate that bile acids contribute to its behavioural activity.