Potato Virus M (PVM) ELISA Kit

Name: Potato Virus M (PVM) ELISA Kit
SKU: DEIAPV16
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

potato leaves, tuber eyes, sprouts, stems

Species Reactivity

Plants

Intended Use

The test can be used to detect PVM in infected potato potato leaves, tuber eyes, sprouts, and stems.

Contents of Kit

##500 wells##
Coating antibody: 0.250 ml, 4°C
Detecting conjugate, alkaline phosphatase: 0.250 ml, 4°C
96-well ELISA plates: 5, Room temperature
Instruction: 1

##1000 wells##
Coating antibody: 0.50 ml, 4°C
Detecting conjugate, alkaline phosphatase: 0.50 ml, 4°C
96-well ELISA plates: 10, Room temperature
Instruction: 1

##5000 wells##
Coating antibody: 2*1.25 ml, 4°C
Detecting conjugate, alkaline phosphatase: 2*1.25 ml, 4°C
96-well ELISA plates: 50, Room temperature
Instruction: 1

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is very high. The virus can be consistently detected in infected plant leaf tissues diluted at 1:2430.

General Description

Potato virus M (PVM), a member of the carlavirus group was found to have a sedimentation coefficient (S20,w) of 157 S, a buoyant density in CsCl of 1.322 g·cm−3, an extinction coefficient, at 260 nm, of 2.88 cm2·mg−1, and a nucleic acid content of 6.0%. The PVM genome is a polyadenylate-containing single-stranded RNA with a molecular weight (MW) of 2.5 × 106. Virus-specific, double-stranded RNA is consistently associated with PVM infection. PVM possesses a single capsid polypeptide with a MW of 35.7 × 103.

Standard Curve

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Real-time feasible model-based crystal size and shape control of crystallization processes

29TH EUROPEAN SYMPOSIUM ON COMPUTER AIDED PROCESS ENGINEERING, PT B

Authors: Szilagyi, Botond; Nagy, Zoltan K.

Abstract

The simultaneous control of crystal size and shape is particularly important in fine chemical and pharmaceutical crystallization. These two quantities influence the dissolution rate and bioavailability of final drug products, and also contribute to the manufacturability and efficiency of downstream operations. Numerous practical issues are associated with the implementation of an industrially relevant crystal size and shape control algorithm, such as the limited number of commercially available measurement devices and tight productivity constraints. Model-based control algorithm is required to address these problems, which, however, brings an additional challenge: the high computational demand of the applicable process simulation. In this work we show that the model predictive control, relying on measurements coming from routinely applied, commercially available process analytical technology (PAT) tools may be feasible. The algorithm is based on GPU accelerated full 2D population balance model (PBM) solution, which does not require external computation units (i.e. cloud computing). The state estimation, which is a crucial part of any robust model-based control system, is carried out by fitting the model, through the re-adjustment of some model parameters, on the measured FBRM count, PVM based mean aspect ratio (AR) and solute concentration, in real time. Artificial neural network (ANN) based soft-sensor is employed to simulate the most likely mean AR of the bivariate crystal size distribution (2D CSD) calculated by the PBM, which is compared then to the mean AR measured by the in-situ imaging tool, as a part of real-time parameter re-adjustment.

Detection of a pneumonia virus of mice (PVM) in an African hedgehog (Atelerix arbiventris) with suspected wobbly hedgehog syndrome (WHS)

VETERINARY MICROBIOLOGY

Authors: Madarame, Hiroo; Ogihara, Kikumi; Kimura, Moe; Nagai, Makoto; Omatsu, Tsutomu; Ochiai, Hideharu; Mizutani, Tetsyuya

Abstract

A pneumonia virus of mice (PVM) from an African hedgehog (Atelerix arbiventris) with suspected wobbly hedgehog syndrome (WHS) was detected and genetically characterized. The affected hedgehog had a nonsuppurative encephalitis with vacuolization of the white matter, and the brain samples yielded RNA reads highly homogeneous to PVM strain 15 (96.5% of full genomic sequence homology by analysis of next generation sequencing). PVM antigen was also detected in the brain and the lungs immunohistochemically. A PVM was strongly suggested as a causative agent of encephalitis of a hedgehog with suspected WHS. This is a first report of PVM infection in hedgehogs. (C) 2014 Elsevier B.V. All rights reserved.