Product Overview
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The PRKACB knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
Alternative Names
PRKACB; protein kinase, cAMP-dependent, catalytic, beta; PKACB; cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta; protein kinase A catalytic subunit beta; cAMP-dependent protein kinase catalytic beta subunit isoform 4ab;
Application Notes
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Dilution
Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
Concentration
The protein concentration was determined with BCA assay.
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.
Citations
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