Magic™ Anti-PIN1 (N-termianl)(phospho S16) polyclonal antibody

Coordinated cell polarization in developing tissues is a recurrent theme in multicellular organisms. In plants, a directional distribution of the plant hormone auxin is at the core of many developmental programs. A feedback regulation of auxin on the polarized localization of PIN auxin transporters in individual cells has been proposed as a self-organizing mechanism for coordinated tissue polarization, but the molecular mechanisms linking auxin signalling to PIN-dependent auxin transport remain unknown. We used a microarray-based approach to find regulators of the auxin-induced PIN relocation in Arabidopsis thaliana root, and identified a subset of a family of phosphatidylinositol transfer proteins (PITPs), the PATELLINs (PATLs). Here, we show that PATLs are expressed in partially overlapping cell types in different tissues going through mitosis or initiating differentiation programs. PATLs are plasma membrane-associated proteins accumulated in Arabidopsis embryos, primary roots, lateral root primordia and developing stomata. Higher order patl mutants display reduced PIN1 repolarization in response to auxin, shorter root apical meristem, and drastic defects in embryo and seedling development. This suggests that PATLs play a redundant and crucial role in polarity and patterning in Arabidopsis.

Farnesene is a sesquiterpene with semiochemical activity involved in interspecies communication. This molecule, known for its phytotoxic potential and its effects on root morphology and anatomy, caused anisotropic growth, bold roots and a "left-handedness" phenotype. These clues suggested an alteration of auxin distribution, and for this reason, the aim of the present study was to evaluate its effects on: i) PIN-FORMED proteins (PIN) distribution, involved in polar auxin transport; ii) PIN genes expression iii) apical meristem anatomy of primary root, in 7 days old Arabidopsis thaliana seedlings treated with farnesene 250 mu M. The following GFP constructs: pSCR::SCR-GFP, pDR5::GFP, pPIN1::PIN1-GFP, pPIN2::PIN2-GFP, pPIN3::PIN3-GFP, pPIN4::PIN4-GFP and pPIN7::PIN7-GFP were used to evaluate auxin distribution. Farnesene caused a reduction in meristematic zone size, an advancement in transition zone, suggesting a premature exit of cells from the meristematic zone, a reduction in cell division and an impairment between epidermal and cortex cells. The auxin-responsive reporter pDR5::GFP highlighted that auxin distribution was impaired in farnesene-treated roots, where auxin distribution appeared maximum in the quiescent center and columella initial cells, without extending to mature columella cells. This finding was further confirmed by the analysis on PIN transport proteins distribution, assessed on individual constructs, which showed an extreme alteration mainly dependent on the PIN 3, 4 and 7, involved in pattern specification during root development and auxin redistribution. Finally, farnesene treatment caused a down-regulation of all the auxin transport genes studied. We propose that farnesene affected auxin transport and distribution causing the alteration of root meristem, and consequently the left-handedness phenotype.