Anti-PIN1 monoclonal antibody, clone 966DU2.8.6

The dopamine transporter (DAT) is a plasma membrane phosphoprotein that actively translocates extracellular dopamine (DA) into presynaptic neurons. The transporter is the primary mechanism for control of DA levels and subsequent neurotransmission, and is the target for abused and therapeutic drugs that exert their effects by suppressing reuptake. The transport capacity of DAT is acutely regulated by signaling systems and drug exposure, providing neurons the ability to fine-tune DA clearance in response to specific conditions. Kinase pathways play major roles in these mechanisms, and this review summarizes the current status of DAT phosphorylation characteristics and the evidence linking transporter phosphorylation to control of reuptake and other functions. Greater understanding of these processes may aid in elucidation of their possible contributions to DA disease states and suggest specific phosphorylation sites as targets for therapeutic manipulation of reuptake. (C) 2016 Published by Elsevier B.V.

Polyphasic taxonomic analysis was performed to characterize a novel bacterium, which was isolated from surface sediment of the Terra Nova Bay, Antarctica, and designated as R04H25(T). The cells of the isolate were Gram-stain-negative, aerobic, nonmotile, slightly curved rods. Growth occurred at 4-42 degrees C, pH 7.0-9.5, and in 1-15% (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences indicated that strain R04H25(T) formed an independent lineage within the genus Pseudidiomarina and its nearest neighbours were Pseudidiomarina donghaiensis 908033(T) (98.2%), Pseudidiomarina marina PIM1(T) (98.1%), Pseudidiomarina woesei W11(T) (97.8%), Pseudidiomarina maritima 908087 (97.1%) and Pseudidiomarina tainanensis PIN1(T) (97.0%). The average nucleotide identities between strain R04H25(T) and the nearest neighbours were 76.2-77.7%. The major fatty acids were iso-C-17:0, summed feature 9, iso-C-15:0,C-16:0 and iso-C-11:0 3-OH. The polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminophospholipid, three unidentified glycolipids and two unidentified lipids. The predominant respiratory quinone was ubiquinone 8. The genomic DNA G+C content was 48.2 mol%. On the basis of the phylogenetic, physiological and chemotaxonomic results, we propose a novel species named as Pseudidiomarina gelatinasegens sp. nov. in the genus Pseudidiomarina, with the type strain R04H25(T) (=GDMCC 1.1503(T) =KCTC 62911(T)).