PDK1 Knockout Cell Lysate from HeLa cell line for anti-PDK1 antibody specificity validation.
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The PDK1 knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
PDK1; pyruvate dehydrogenase kinase, isozyme 1; pyruvate dehydrogenase kinase, isoenzyme 1; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
The protein concentration was determined with BCA assay.
Store at -20°C. Avoid repeated freeze-thaw cycles.