Osteopontin Mouse ELISA Kit (DEIA1585)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
cell culture supernatants, serum, plasma
Species Reactivity
Mouse
Intended Use
For the quantitative measurement of Mouse Osteopontin concentrations in serum, plasma and cell culture supernatants.
Contents of Kit
1. Osteopontin Microplate (Item A)
2. Wash Buffer Concentrate (20x) (Item B)
3. Standards (Item C)
4. Assay Diluent C (Item L) (For Standard/Sample (serum/plasma samples/cell culture medium) diluent)
5. Assay Diluent B (Item E) (For detection antibody and HRP-Streptavidin diluent)
6. Detection Antibody Osteopontin (Item F)
7. HRP-Streptavidin concentrate (Item G)
8. TMB One-Step Substrate Reagent (Item H)
9. Stop Solution (Item I)
Storage
This kit may be stored for up to 6 months at 2-8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be stored for up to 1 month at 2-8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Precision
Intra-assay: CV<10%
Inter-Assay: CV<12%
Detection Range
2.74 pg/mL - 2000 pg/mL
Sensitivity
8 pg/mL
Standard Curve

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