Osteopontin Mouse ELISA Kit (DEIA1585)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Summary
Documentation

Size

96T

Sample

cell culture supernatants, serum, plasma

Species Reactivity

Mouse

Intended Use

For the quantitative measurement of Mouse Osteopontin concentrations in serum, plasma and cell culture supernatants.

Contents of Kit

1. Osteopontin Microplate (Item A)
2. Wash Buffer Concentrate (20x) (Item B)
3. Standards (Item C)
4. Assay Diluent C (Item L) (For Standard/Sample (serum/plasma samples/cell culture medium) diluent)
5. Assay Diluent B (Item E) (For detection antibody and HRP-Streptavidin diluent)
6. Detection Antibody Osteopontin (Item F)
7. HRP-Streptavidin concentrate (Item G)
8. TMB One-Step Substrate Reagent (Item H)
9. Stop Solution (Item I)

Storage

This kit may be stored for up to 6 months at 2-8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be stored for up to 1 month at 2-8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Precision

Intra-assay: CV<10%
Inter-Assay: CV<12%

Detection Range

2.74 pg/mL - 2000 pg/mL

Sensitivity

8 pg/mL

Standard Curve