Ochratoxin-A ELISA Kit

Name: Ochratoxin-A ELISA Kit
SKU: DEIA4010
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Animal Feed, Cereal, Coffee, Grain

Detection Method

sELISA

Intended Use

The Ochratoxin-A ELISA Kit is intended for the quantitative detection of ochratoxin-A levels in grains, cereals, coffee, and other commodities including animal feeds.

Contents of Kit

Storage

All reagents of the kit are stable, if stores at 2 - 8°C, until the expiration date stated on the kit.

General Description

Ochratoxin-A is a toxic metabolite produced by several molds of the Aspergillus flavus and Penicillium genera, including Aspergillus ochraceus. The fungal species has the potential to produce ochratoxin-A, a known nephrotoxin and carcinogen. It has been frequently detected in human foods and animal feed, mainly in cereal products, although a range of commodities has been reported to contain the toxin. In humans, exposure to ochratoxin-A has been linked with Balken endemic nephropathy (BEN), a chronic kidney disease associated with tumors of the renal system. In animals, impairment of renal function has been reported in swine. In turkeys and chickens symptoms included retarded growth, decreased feed conversion, nephropathy and mortality. Feed refusal has also been observed in turkeys. A decrease in egg production and shell quality was reported in both turkeys and chickens.

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

A power efficient low-noise source degenerated bio-potential amplifier

ANALOG INTEGRATED CIRCUITS AND SIGNAL PROCESSING

Authors: Venishetty, Sudheer Raja; Kumaravel, S.; Durai, S. Ananiah

Abstract

In literature double recycling folded cascode operational transconductance amplifier (DRFC-OTA) is realized to achieve higher transconductance. To realize preamplifier for bio-medical applications, it is necessary to design an OTA with lower noise. In this paper source degeneration is adopted for DRFC-OTA for further reduction of noise and is proposed. To access the efficacy of this technique, the preamplifier using DRFC-OTA with source degeneration (DRFCSD) is realized in UMC 180 nm technology and is studied through simulations. For sizing the transistors of DRFCSD, gm/ID methodology is adopted. From the simulation results it is observed that, the preamplifier exhibits a mid-band gain of 40.02 dB at - 3 dB bandwidth of 5.69 Hz-5.45 kHz. The total input referred noise of amplifier is found to be 3.27 mu Vrms for 5.69 Hz-5.45 kHz for a power consumption of 1.53 mu W. The noise efficiency factor is found to be 1.58 and provides an output swing of 1.2 Vpp at THD of 1 %. CMRR and PSRR of the preamplifier are simulated and found to be 66.55 dB and 54.99 dB respectively. The amplifier occupies an area of 0.16mm(2).

Fungal Diversity and Evaluation of Ochratoxin A Content of Coffee from Three Cameroonian Regions

JOURNAL OF FOOD QUALITY

Authors: Nganou, N. D.; Tchinda, E. S.; Noumo, T. N.; Mouafo, H. T.; Sokamte, A. T.; Tatsadjieu, L. N.

Abstract

The present study had the objective to assess the ochratoxin A content of coffee through chromatographic analysis and design a method using PCR-DGGE to analyze at the same moment the totality of fungal flora present in the coffee samples in order to determine their geographic origin. 96 samples of coffee were collected from the west region (Bafoussam and Dschang), centre region (Bafia), and east region (Batouri) of Cameroon during two years (2017 and 2018). Two treatments (dry and wet routes) were evaluated at three different steps of coffee processing (parchment coffee, green coffee, and husk coffee). The characterization of the fungal profile was done with PCR-DGGE and sequencing. The levels of OTA were assessed using HPLC analysis. The results indicated that the toxinogenic mycoflora associated with coffee beans was mainly Aspergillus niger, A. carbonarius, and A. ochraceus. PCR-DGGE data revealed that each sampling site is characterized by a specific fungal profile. Despite the influence of the treatment on the fungal population of coffee, bands common to samples coming from the same site were observed. These bands could therefore constitute potential biological markers to trace back to the origin of coffee. OTA was detected in most of the coffee samples analyzed and only few samples contented OTA at levels higher than the maximum tolerable limit for food intended for human consumption. The OTA content of coffee was significantly influenced by the sampling step and the sampling period.