Nrf2 ELISA Kit

Name: Nrf2 ELISA Kit
SKU: DEIA-XYA1347
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

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COA

SDS

Datasheet

Sample

cultured cells

Species Reactivity

Human, Mouse, Rat

Detection Method

iELISA

Intended Use

The Nrf2 Colorimetric Cell-Based ELISA Kit allows for the qualitative detection of Nrf2 and the effects that certain stimulation conditions have on Nrf2 expression in different cell lines.

Contents of Kit

1. 96-Well Cell Culture Clear-Bottom Microplate. 1 Plate
2. 10× TBS, 24 mL (10×)
3. Quenching Buffer, 24 mL (1×)
4. Blocking Buffer, 50 mL (1×)
5. 15× Wash Buffer, 50 mL (15×)
6. 100× Anti- Nrf2 Antibody, 60 μL (100×)
7. 100× Anti-GAPDH Antibody, 60 μL (100×)
8. HRP-Conjugated Anti-Rabbit IgG Antibody, 6 mL (1×)
9. HRP-Conjugated Anti-Mouse IgG Antibody, 6 mL (1×)
10. Primary Antibody Diluent, 12 mL (1×)
11. Ready to Use Substrate, 12 mL (1×)
12. Stop Solution, 12 mL (1×)
13. Crystal Violet Solution, 6 mL (1×)
14. SDS Solution, 24 mL (1×)
15. Adhesive Plate Seals, 2 Seals

Storage

4°C/6 Months

Citations

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Certificate of Analysis

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Customer Reviews

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Autophagy controls neonatal myogenesis by regulating the GH-IGF1 system through a NFE2L2-and DDIT3-mediated mechanism

AUTOPHAGY

Authors: Zecchini, Silvia; Giovarelli, Matteo; Perrotta, Cristiana; Morisi, Federica; Touvier, Thierry; Di Renzo, Ilaria; Moscheni, Claudia; Bassi, Maria Teresa; Cervia, Davide; Sandri, Marco; Clementi, Emilio; De Palma, Clara

Abstract

Macroautophagy/autophagy is emerging as an important process in adult muscle stem cells functions: it regulates metabolic reprogramming during activation from a quiescent state, maintains stemness and prevents senescence. We now show that autophagy is specifically required for neonatal myogenesis and muscle development. Specific deletion of Atg7 in PAX7(+) (paired box 7) precursors led in mice to a dwarf phenotype, with an effect restricted to the neonatal phase of muscle development. Atg7 knockdown suppressed neonatal satellite cell (nSC) proliferation and differentiation, downregulating the GH-IGF1 functions. When we disrupted autophagy, NFE2L2/NRF2 (nuclear factor, erythroid 2 like 2) accumulated in muscle and nSCs and negatively modulated DDIT3/CHOP (DNA-damage inducible transcript 3) expression. Lower levels of DDIT3 were responsible for reduced GHR expression leading to impaired local production of IGF1. Our results conclusively identify a novel autophagy-dependent pathway that regulates nSC behavior and indicate that autophagy is required for skeletal muscle development in the neonatal phase.

The NFE2L2 rs35652124 polymorphism and the risk of Parkinson's disease: a systematic review and meta-analysis

NEUROREPORT

Authors: Zhu, Mengru; Zhou, TingTing; Zu, Guo; Liang, ZhanHua

Abstract

The relationship between the nuclear factor erythroid-derived 2-like 2 (NFE2L2) rs35652124 (A/G) polymorphism and the risk of Parkinson's disease (PD) is controversial. To evaluate the association of the NFE2L2 rs35652124 polymorphism with the risk of PD, a meta-analysis was carried out. A total of 2264 PD cases and 2582 controls were included in the meta-analysis on the basis of strict inclusion and exclusion criteria. The pooled odds ratio (OR) and 95% confidence interval were calculated to assess the genetic association between the NFE2L2 rs35652124 polymorphism and the risk of PD. There was no significant association between rs35652124 and PD (G vs. A: OR = 1.001, P = 0.986; GG vs. AA: OR = 1.026, P = 0.785; GA vs. AA: OR = 1.023, P = 0.786; GG + GA vs. AA: OR = 1.224, P = 0.070; GG vs. GA + AA: OR = 0.994, P = 0.926). The data of our meta-analysis indicate that the G allele, GG, and GA genotype of the rs35652124 (A/G) polymorphism were not associated with the risk of PD. Copyright (C) 2016 Wolters Kluwer Health, Inc. All rights reserved.