Nitrofurantoin (AHD) ELISA Kit

Sample

Muscle, honey, milk, etc

Detection Method

cELISA

Intended Use

This kit uses Competitive-ELISA as the method for the quantitative detection. It can detect Nitrofurantoin (AHD) in samples, such as muscle, honey, milk, etc.

Contents of Kit

| ELISA Microtiter plate | 96 wells |
| Standard | Liquid 1 mL each (ppb=ng/mL=ng/g)
(0 ppb,0.02 ppb,0.06 ppb,0.18 ppb,0.54 ppb,1.62 ppb) |
| Derivatization Reagent | 10 mL |
| HRP Conjugate | 5.5 mL |
| Antibody Working Solution | 5.5 mL |
| Substrate Reagent A | 6 mL |
| Substrate Reagent B | 6 mL |
| Stop Solution | 6 mL |
| 20×Concentrated Wash Buffer | 40 mL |
| 2×Reconstitution Buffer | 50 mL |
| Plate Sealer | 3 pieces |
| Sealed Bag | 1 piece |
| Manual | 1 copy |
Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.

Storage

Store the kit at 2-8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2-8°C.

Detection Limit

Muscle (livestock), Liver, Honey, Milk ---0.04 ppb;
Milk powder, Feed, Egg powder, Egg---0.04 ppb;
Muscle (fish, shrimp) ---0.06 ppb