Sample
cell culture supernatants, urine, serum, plasma
Detection Method
Sandwich ELISA
Intended Use
For the quantitative measurement of Human Neurotrophin 3 concentrations in cell and tissuelysates.
Contents of Kit
1. Neurotrophin 3 Microplate (Item A)
2. Wash Buffer Concentrate (20x) (Item B)
3. Standards (Item C)
4. Assay Diluent A (Item D) (for Standard/Sample (serum/plasma) diluent)
5. Assay Diluent B (Item E) (for Standard/Sample (cell culture medium/urine) diluent)
6. Detection Antibody Neurotrophin 3 (Item F)
7. HRP-Streptavidin concentrate (Item G)
8. TMB One-Step Substrate Reagent (Item H)
9. Stop Solution (Item I)
Storage
All kit components of this kit are stable at 2 to 8°C. For more detailed information, please download the following document on our website.
Detection Range
4.12 pg/mL - 3000 pg/mL
Citations
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WILSON, C; ANGLMAYER, R; et al. MOLECULAR-CLONING, FUNCTIONAL EXPRESSION IN ESCHERICHIA-COLI, AND CHARACTERIZATION OF MULTIPLE MITOGEN-ACTIVATED-PROTEIN KINASES FROM TOBACCO. EUROPEAN JOURNAL OF BIOCHEMISTRY 233:249-257(1995).
Clement, TM; Bhandari, RK; et al. SRY Directly Regulates the Neurotrophin 3 Promoter During Male Sex Determination and Testis Development in Rats. BIOLOGY OF REPRODUCTION 85:277-284(2011).