Natamycin ELISA Kit

Sample

Meat/Tissue /Fish/Shrimp/Milk Powder/Infant Formula/Pure Milk/Fresh Milk/Egg

Detection Method

cELISA

Intended Use

Natamycin ELISA Test Kit enables government agencies, food manufacturers and quality assurance organizations to detect Natamycin to as low as 0.1 ug/g level in various sample types and to satisfy customer concerns about food safety.
The unique features of the kit:
The extraction of Natamycin from meat/tissue with a high recovery rate.
A quick ELISA assay (less than 1 hours regardless of number of samples).
High reproducibility Procedure Overview.

Contents of Kit

Natamycin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package.
1. Natamycin Coated Plate, 1 x 96-well Plate (8 wells x 12 strips)
2. Natamycin Standards:(each of 1mL)
Negative control (white cap tube)
0.1 ug/mL (yellow cap tube)
0.25 ug/mL (orange cap tube)
0.5 ug/mL (pink cap tube)
1.0 ug/mL (purple cap tube)
5.0 ug/mL (blue cap tube)
100ug/mL (spiking, optional, red cap tube)
3. 10 X Natamycin Antibody 1#, 0.8 mL
4. Antibody Solution, 10 mL
5. HRP Conjugate Antibody 2#, 6.0 mL
6. 20X Wash Solution, 30 mL
7. Stop Buffer, 12 mL
8. 10 X Sample Extraction Buffer, 30 mL
9. TMB Substrate, 12 mL
Note: If you are not planning to use the kit for over 1 month, store Natamycin Standards, Natamycin Antibody and HRP-Conjugate at -20°C or in a freezer.

Storage

Store the kit at 2-8°C . The shelf life is 12 months when the kit is properly stored.

Sensitivity

Fresh milk, 0.1ug/g
Egg/Pure milk, 1 ug/g
Milk powder/Infant Formula, 2 ug/g
Meat/Tissue/Fish/Shrimp,0.2 ug/g