Influenza A Virus Nucleoprotein Antigen ELISA Kit

Sample

Influenza Virus Sample Inactivation and Lysis

Species Reactivity

IAV

Detection Method

sELISA

Intended Use

CD Influenza A nucleoprotein ELISA Kit is an enzyme immunoassay developed for detection and quantitation of the Influenza A nucleoprotein. The ELISA antibodies only recognize the nucleoprotein from Influenza A, and will not react with the nucleoprotein from Influenza B nor Influenza C. The kit has a detection sensitivity limit of 625 pg/mL Influenza A nucleoprotein. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and Influenza A lysate samples

Contents of Kit

Box 1 (shipped at room temperature)
1. Anti-Influenza A Nucleoprotein Antibody Coated Plate: One strip well 96-well plate.
2. FITC-Conjugated Anti-Influenza A Nucleoprotein Monoclonal Antibody: One 20 μL vial.
3. HRP-Conjugated Anti-FITC Monoclonal Antibody: One 20 μL vial.
4. Assay Diluent: One 50 mL bottle.
5. 10× Viral Lysis Buffer: One 15 mL bottle containing 200 mM Tris, pH 7.5, 1500 mM NaCl, 10% Triton X-100, 1% SDS.
6. 10× Wash Buffer: One 100 mL bottle.
7. Substrate Solution: One 12 mL amber bottle.
8. Stop Solution: One 12 mL bottle.
Box 2 (shipped on blue ice packs)
1. Recombinant Influenza A Nucleoprotein Standard: One 100 μL vial of 4 μg/mL recombinant human Influenza B nucleoprotein (Met1-Tyr560) in PBS containing BSA.

Storage

Upon receiving, aliquot and store the Influenza A Nucleoprotein Standard at -20°C and avoid freeze/thaw. Store all other components at 4°C.

Sensitivity

0.625 ng/mL

General Description

The influenza virus is an enveloped virus that can be divided into three classes, A, B, and C, largely based upon conserved antigenic differences in the internal nucleoprotein (NP). Only Influenza A and B are clinically relevant for humans. Influenza A virus, typically encountered more frequently than type B, is associated with the majority of serious epidemics, and it can be further subdivided into strains or subtypes based on antigenic differences in the external hemagglutinin proteins (H1-H16) and neuraminidase proteins (N1-N9).
The primary function of NP is to encapsidate the segmented RNA and bind with the three polymerase subunits, PA, PB1 and PB2, to form ribonucleoprotein particles (RNPs) for RNA transcription, replication and packaging.