Product Overview
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The NOTCH1 knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
Alternative Names
NOTCH1; notch 1; N1; Mis6; Tan1; lin-12; 9930111A19Rik; neurogenic locus notch homolog protein 1; Motch A; Notch gene homolog 1; mT14; major type A protein; p300; transmembrane receptor Notch1;
Application Notes
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Dilution
Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
Concentration
The protein concentration was determined with BCA assay.
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.
Citations
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Rennstam, K; McMichael, N; et al. Numb protein expression correlates with a basal-like phenotype and cancer stem cell markers in primary breast cancer. BREAST CANCER RESEARCH AND TREATMENT 122:315-324(2010).
Abdel-Rahman, N; Martinez-Arias, A; et al. Probing the druggability of protein-protein interactions: targeting the Notch1 receptor ankyrin domain using a fragment-based approach. BIOCHEMICAL SOCIETY TRANSACTIONS 39:1327-1333(2011).
COA
Certificate of Analysis
