NME2 Knockout Cell Lysate

Name: NME2 Knockout Cell Lysate
SKU: DAG-KO337
Rating: 5 (1 reviews)

NME2 Knockout Cell Lysate from HeLa cell line for anti-NME2 antibody specificity validation.

COA

SDS

Datasheet

Summary

Product Overview

HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The NME2 knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.

Nature

Cell Lysate

Alternative Names

NME2; non-metastatic cells 2, protein (NM23B) expressed in; nucleoside diphosphate kinase B; NM23 H2; NDP kinase B; c-myc transcription factor; histidine protein kinase NDKB; nucleotide diphosphate kinase B; c-myc purine-binding transcription factor PUF; non-metastatic cells 2, protein (NM23) expressed in; PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2; MGC2212; MGC111212;

Application Notes

Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Dilution

Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.

Format

Lyophilized

Concentration

The protein concentration was determined with BCA assay.

Buffer

RIPA buffer

Preservative

None

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Citations

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Documents

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pdf Datasheet

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References

Substituent effects on gas-phase homolytic Fe-N bond energies of m-G-C6H4NHFe(CO)(2)((5)-C5H5) and m-G-C6H4N(COMe)Fe(CO)(2)((5)-C5H5) studied using density functional theory methods

JOURNAL OF PHYSICAL ORGANIC CHEMISTRY

Authors: Zeng, Qing; Li, Zucheng; Wang, Yi-Bo

Abstract

One of the most fundamental properties in chemistry is the bond dissociation energy, the energy required to break a specific bond of a molecule. In this paper, the Fe-N homolytic bond dissociation energies [H-homo(Fe-N)'s] of 2 series of (meta-substituted anilinyl)dicarbonyl((5)-cyclopentadienyl) iron [m-G-C(6)H(4)NHFp (1)] and (meta-substituted -acetylanilinyl)dicarbonyl((5)-cyclopentadienyl) iron [m-G-C6H4N(COMe)Fp (2)] were studied using density functional theory methods with large basis sets. In this study, Fp is ((5)-C5H5)Fe(CO)(2), and G is NO2, CN, COMe, CO2Me, CF3, Br, Cl, F, H, Me, MeO, and NMe2. The results show that Tao-Perdew-Staroverov-Scuseria, Minnesota 2006, and Becke's power-series ansatz from 1997 with dispersion corrections functionals can provide the best price/performance ratio and accurate predictions of H-homo(Fe-N)'s. The H-homo(Fe-N)'s (1 and 2) conform to the captodative principle. The polar effects of the meta-substituents show the dominant role to the magnitudes of H-homo(Fe-N)'s. sigma and sigma(c) values for meta-substituents are all related to polar effects. Spin-delocalization effects of the meta-substituents in H-homo(Fe-N)'s are small but not necessarily zero. RE plays an important role in determining the net substituent effects on H-homo(Fe-N)'s. Insight from this work may help the design of more effective catalytic processes.

Oligogermanes Containing Only Electron-Withdrawing Substituents: Synthesis and Properties

ORGANOMETALLICS

Authors: Zaitsev, Kirill V.; Lam, Kevin; Zhanabil, Zhaisan; Suleimen, Yerlan; Kharcheva, Anastasia V.; Tafeenko, Viktor A.; Oprunenko, Yuri F.; Poleshchuk, Oleg Kh.; Lermontova, Elmira Kh.; Churakov, Andrei V.

Abstract

A series of germanes Ar3GeX, containing electron-withdrawing substituents [Ar = p-FC6H4, 1a-d, 1a (X = CO, 1b (X = Br), 1c (X = H), 1d (X = NMe2); p-F3CC6H4, 2a-d, 2a (X = Cl), 2b (X = Br), 2c (X = H), 2d (X = NMe2)], was synthesized and used to prepare symmetrical digermanes Ar3Ge-GeAr3, (p-FC6H4)(3)GeGe(C6H4F-p)(3) (3), and (p-F3CC6H4)(3)GeGe(C6H4CF3-p)(3) (4) and trigermane [(p-F3CC6H4)(3)Ge](2)Ge(C6F5)(2) (5) by hydrogermolysis reaction. The properties of all compounds were investigated by multinuclear NMR and for oligogermanes by UV/vis and fluorescence spectroscopy, as well as by electrochemical methods. In addition, the molecular structures of 1a, 1b, 2b, 2c, and 3-5 were studied by X-ray diffraction analysis. Compound 5 showed a significantly shifted UV/visible absorption to the red field in comparison with previously described derivatives.