Sample
Serum, plasma, tissue homogenates and other biological fluids
Storage
Open kit immediately upon receipt. Store components at -20°C (NOTE: exceptions below) for 6 months or until expiration date. Avoid any freeze/thaw cycles.
Precision
Three sample’s concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Inter-Assay precision.
Mean Intra-Assay Precision- ≤ 6.5%, n = 20
Mean Inter-Assay Precision- ≤ 9.7%, n = 20
Detection Range
31.2 - 2000 pg/mL
Detection Limit
< 10 pg/mL (Derived by linear regression of OD450 of the Mean Blank + 2xSD)
General Description
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins and, like the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments.
Citations
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Murray, KD; Rubin, CM; et al. Molecular Correlates of Laminar Differences in the Macaque Dorsal Lateral Geniculate Nucleus. JOURNAL OF NEUROSCIENCE 28:12010-12022(2008).
Fiordelisio, T; Millan-Aldaco, D; et al. Cells of Proopiomelanocortin Lineage from the Rodent Anterior Pituitary Lack Sexually Dimorphic Expression of Neurofilaments. NEUROENDOCRINOLOGY 83:360-370(2006).
COA
Certificate of Analysis
