Sample
cell culture supernatants, serum, plasma
Species Reactivity
Mouse; Rat
Detection Method
Sandwich ELISA
Intended Use
For the quantitative determination of mouse or rat Platelet-Derived Growth Factor BB (PDGF-BB) concentrations in cell culture supernates, serum, and plasma.
Contents of Kit
1. Mouse/Rat PDGF-BB Microplate
2. Mouse/Rat PDGF-BB Conjugate
3. Mouse/Rat PDGF-BB Standard
4. Mouse/Rat PDGF-BB Control
5. Assay Diluent: 11 mL
6. Calibrator Diluent A
7. Calibrator Diluent B
8. Wash Buffer Concentrate
9. Color Reagent A
10. Color Reagent B
11. Stop Solution
Storage
Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles.
Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2-8°C.
Precision
Cell Culture Supernate Assay
Intra-assay Precision: 4.5-9.0%
Inter-assay Precision: 5.0-10.2%
Serum/Plasma Assay
Intra-assay Precision: 4.0-9.2%
Inter-assay Precision: 8.6-9.5%
Detection Range
4.0-19.3 pg/mL
Citations
Publication ()
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