Specifications
Immunogen
Native purified CD8B from normal human T cells.
Applications
Application Notes
Flow Cytometry (2ug/5x105 cells/test or 100μl of whole blood); Immunofluorescence (2ug/5x105 cells/test or 100μl of whole blood) & The optimal working dilution should be determined by the end user.
Target
Alternative Names
MGC119115; P37; CD8 antigen, beta polypeptide 1 (p37); T lymphocyte surface glycoprotein beta chain; LEU2; T-cell surface glycoprotein CD8 beta chain; LY3; LYT3; CD8B1; OTTHUMP00000160762; OTTHUMP00000160763; OTTHUMP00000198673; OTTHUMP00000203521
Product Background
Antigen Description
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.
Mouse monoclonal antibody raised against native CD8B.
Pathway
Adaptive Immunity Signaling, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem
Citations
Publication ()
Have you cited CABT-24546MH in a publication?
Let us know and earn a reward for your research.
Enhancing Efficacy of TCR-engineered CD4 + T Cells Via Coexpression of CD8α
Victoria E Anderson, Sara S Brilha, Anika M Weber, Annette Pachnio, Guy E Wiedermann, Sumaya Dauleh, Tina Ahmed, George R Pope, Laura L Quinn, Roslin Y Docta, Adriano Quattrini, Siobhan Masters, Neil Cartwright, Preetha Viswanathan, Luca Melchiori, Louise V Rice, Alexandra Sevko, Claire Gueguen, Manoj Saini, Barbara Tavano, Rachel J M Abbott, Jonathan D Silk, Bruno Laugel, Joseph P Sanderson, Andrew B Gerry
Applications: FC
Reactive species: Human
"Abstract: Adoptive cell therapy with T cells expressing affinity-enhanced T-cell receptors (TCRs) is a promising treatment for solid tumors. Efforts are ongoing to further engineer these T cells to increase the depth and durability of clinical responses and broaden efficacy toward additional indications. In the present study, we investigated one such approach: T cells were transduced with a lentiviral vector to coexpress an affinity-enhanced HLA class I-restricted TCR directed against MAGE-A4 alongside a CD8α coreceptor. We hypothesized that this approach would enhance CD4 + T-cell helper and effector functions, possibly leading to a more potent antitumor response. Activation of transduced CD4 + T cells was measured by detecting CD40 ligand expression on the surface and cytokine and chemokine secretion from CD4 + T cells and dendritic cells cultured with melanoma-associated antigen A4 + tumor cells. In addition, T-cell cytotoxic activity against 3-dimensional tumor spheroids was measured. Our data demonstrated that CD4 + T cells coexpressing the TCR and CD8α coreceptor displayed enhanced responses, including CD40 ligand expression, interferon-gamma secretion, and cytotoxic activity, along with improved dendritic cell activation. Therefore, our study supports the addition of the CD8α coreceptor to HLA class I-restricted TCR-engineered T cells to enhance CD4 + T-cell functions, which may potentially improve the depth and durability of antitumor responses in patients."
Article snippet: Purified CD8+ and CD4+ populations were obtained by negative selection with either CD8β antibody (clone 3TU9618; Creative Diagnostics) and anti-mouse immunoglobulin G beads or anti-CD4+ microbeads (*), according to the manufacturer’s instructions. 
Figure 1. Scatter plots showing spheroid area under the curve with isolated CD4+ (left) or CD8+ (center) T-cell fractions, or unseparated T cells (right)
COA
Loading ......
