Guinea pig Lipid peroxide ELISA Kit

Oxidative stress parameters were evaluated during the first 72 h of embryonic development of purple sea urchin Strongylocentrotus purpuratus continuously exposed to control conditions, to cadmium alone (Cd, 30 mu g/L), to zinc alone (Zn, 9 mu g/L) or to a Cd (28 mu g/L) plus Zn (9 mu g/L) mixture. These sublethal concentrations represent similar to 10% of the acute EC50. Bioaccumulation, antioxidant capacity against peroxyl radicals (ACAP), total glutathione (GSH) level, glutathione-S-transferase (GST), glucose-6-phosphate dehydrogenase (G6PDH) and superoxide dismutase (SOD) activity, and lipid peroxidation (LPO) were analyzed at 24 h (blastula), 48 h (gastrula), and 72 h (plutcus) stages of development. Zinc (an essential metal) was well-regulated, whereas Cd (non-essential) bioaccumulated and whole-body [Cd] increased from blastula to pluteus stage in sea urchin larvae. In controls, ACAP progressively declined from 24 h to 72h, while LPO reciprocally increased, but other parameters did not change. Cd alone was more potent than Zn alone as a pro-oxidant, with the major effects being decreases in SOD activity and parallel increases in LPO throughout development; GST activity also increased at 24 h. Zn alone caused only biphasic disturbances of ACAP. In all cases, the simultaneous presence of the other metal prevented the effects, and there was no instance where the oxidative stress response in the presence of the Cd/Zn mixture was greater than in the presence of either Cd or Zn alone. Therefore the sublethal effects of joint exposures were always less than additive or even protective, in agreement with classical toxicity data. Furthermore, our results indicate that SOD and Zn can play important roles in protecting sea urchin embryos against Cd-induced lipid peroxidation.

The aim of this study was to evaluate the effect of diets containing different dried by-products on milk and blood plasma antioxidant capacity of dairy ewes. Thirty-six Sarda ewes were assigned to four treatments: control (CON; no by-product), 100 g/day of grape marc (GM), 100 g/day tomato pomace (TP) and 75 g/day of exhausted myrtle berries (EMBs). The superoxide dismutase (SOD), glutathione reductase (GR), glutathione transferase (GST) and glutathione peroxidase (GSH-Px) in blood, and SOD, GR and lactoperoxidase (LPO) in milk were determined. Total antioxidant capacity (FRAP and ABTS assays), malondialdehyde (MDA) and protein carbonyls (PCs) were also measured. Milk fatty acid profile was investigated by gas chromatography. The results showed higher antioxidant capacity measured by FRAP or ABTS assays and a reduction in MDA in GM plasma than CON. All by-products enhanced the protection of milk proteins by oxidation, as evidenced by lower values of PCs compared with CON. GM supplementation increased PUFAn-6, due to increase in C18:2n-6, the main component of GM compared with CON. All by-products did not modify the nutritional indexes of milk fat. In conclusion, dietary GM may enhance protection against oxidative condition of dairy ewes, whereas TP and EMB need further research to define the optimum inclusion level in sheep diet.