Sample
Serum, plasma, cell culture supernatant and other biological samples
Species Reactivity
Bovine
Intended Use
In vitro quantitative determination of IPTH concentrations in serum, plasma, cell culture supernatant and other biological samples.
Contents of Kit
The sealed kit can be stored at 2-8 °C. The storage condition for opened kit is specified in the table below:
1. ELISA Microplate(Dismountable), 8×12. Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C.
2. Lyophilized Standard, 2vial. Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C
3. Biotin-labeled Antibody(Concentrated, 100×), 120μl. 2-8°C (Avoid Direct Light).
4. HRP-Streptavidin Conjugate(SABC, 100×), 120μl. 2-8°C (Avoid Direct Light).
5. TMB Substrate, 10ml. 2-8°C (Avoid Direct Light).
6. Sample Dilution Buffer, 20ml. 2-8°C.
7. Antibody Dilution Buffer, 10ml. 2-8°C.
8. SABC Dilution Buffer, 10ml. 2-8°C.
9. Stop Solution, 10ml. 2-8°C.
10. Wash Buffer(25×), 30ml. 2-8°C.
11. Preprocess Agent A, 1.5ml. 2vial. 2-8°C.
12. Preprocess Agent B, 1.5ml. 2vial. 2-8°C.
13. Plate Sealer, 5 pieces
14. Product Description, 1 copy
Note: The liquid reagent bottle contains slightly more reagent than indicated on the label. Please use pipette accurately measure and do proportional dilution.
Storage
2-8°C (for sealed box), please do not freeze! See kit label for expiry date
Performance Characteristics
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Detection Range
15.625-1000pg/ml
General Description
Uniprot ID: P01268
Parathyroid hormone (PTH) is a hormone that parathyroid glands make and release to control the level of calcium in the blood, not in the bones. Calcium is one of the most important and common minerals in body. PTH also helps control the levels of phosphorus and vitamin D in blood and bones.
Standard Curve
This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C -25°C. TMB was balanced to 37°C before color development, and incubated at 37°C for 15 minutes in the dark after adding the enzyme label plate holes.)
The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.
General Notes
Uniprot ID: P01268
Parathyroid hormone (PTH) is a hormone that parathyroid glands make and release to control the level of calcium in the blood, not in the bones. Calcium is one of the most important and common minerals in body. PTH also helps control the levels of phosphorus and vitamin D in blood and bones.
Citations
Publication ()
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Effect of postpartum calcium supplementation on serum calcium and parathyroid hormone concentrations in multiparous Holstein cows
Frost IR, Seely CR, McCray HA, Callero KR, Seminara JA, Martinez RM, Reid AM, Wilbur CN, Koebel KJ, McArt JAA.
Applications: Unspecified application
Reactive species: Unspecified reactive species
"Abstract: Although postpartum Ca supplementation strategies are often employed to prevent subclinical hypocalcemia in dairy cows, these strategies have produced a mix of beneficial, neutral, and detrimental results when assessing milk yield and subsequent disease outcomes. Because the mechanisms underlying these differing results are unknown, our objectives were to determine how common postpartum Ca supplementation strategies affect blood Ca concentrations and parathyroid hormone (PTH). We conducted a randomized controlled trial with 74 multiparous dairy cows on a commercial dairy in central New York. Cows were assigned to 1 of 4 supplementation groups immediately after calving: (1) control (CON; no Ca supplementation, n = 15); (2) conventional oral Ca supplementation (BOL-C; 43 g of oral Ca bolus administered immediately after calving and 24 h later, n = 17); (3) delayed oral Ca supplementation (BOL-D; 43 g of oral Ca bolus administered 48 and 72 h after calving, n = 15); or (4) subcutaneous infusion (SQ; 500 mL of 23% Ca borogluconate infused subcutaneously once immediately after calving, n = 15). Blood samples were collected immediately after calving (0 h) and at 8, 16, 24, 32, 40, 48, 56, 64, 72, 80, 88, 96, 120, and 168 h postpartum for a total of 15 blood samples per cow. Cows were excluded if administered Ca, via any route, by farm employees or if they died or were sold within 96 h following parturition, which left 62 cows for analysis. Linear mixed models, accounting for repeated measures, were created to analyze changes in serum total Ca (tCa) and PTH over the first 168 h after parturition and assess differences between supplementation groups. Serum tCa and PTH concentrations were not different at the time of calving among supplementation groups."
Article snippet: Serum samples were analyzed for PTH by the first author (IRF) using Bovine Intact PTH ELISA kits (DEIA1826B, Creative Diagnostics) following the manufacturer's instructions as previously validated by Connelly et al. (2021). Inter- and intra-assay coefficients of variation were 13.0% and 2.9%, respectively.
Effect of duration of exposure to diets differing in dietary cation-anion difference on Ca metabolism after a parathyroid hormone challenge in dairy cows
Vieira-Neto A, Leão IMR, Prim JG, Silva ACM, Nehme Marinho M, Zimpel R, Etheve S, Nelson CD, Santos JEP
Applications: ELISA
Reactive species: Cows
"Abstract: Objectives of the experiment were to determine the length of exposure to an acidogenic diet that would elicit changes in acid-base balance, mineral digestion, and response to parathyroid hormone (PTH)-induced changes in blood Ca and vitamin D3 in prepartum dairy cows. Nonlactating parous Holstein cows (n = 20) at 242 d of gestation were blocked by lactation (1 or >1) and pretreatment dry matter (DM) intake and, within block, they were randomly assigned to a diet with a dietary cation-anion difference (DCAD) of +200 mEq/kg of DM (DCAD +200) or an acidogenic diet with -150 mEq/kg of DM (DCAD -150). Water and DM intake were measured and blood was sampled daily. Urine was sampled every 3 h for 36 h, and then daily. During PTH challenges on d 3, 8, and 13, cows received i.v. PTH 1-34 fragment at 0.05 µg/kg of body weight every 20 min for 9 h to mimic the pulsatile release of endogenous PTH. Blood was sampled at 0 h, and hourly thereafter until 10 h, and at 12, 18, 24, 36, and 48 h relative to each challenge. Acid-base measures and concentrations of ionized Ca (iCa) in whole blood, and total Ca, Mg, P, and vitamin D metabolites in plasma were evaluated. On d 2 and 7, Ca, Mg, and P balances were evaluated. Cows fed DCAD -150 had smaller blood pH (7.431 vs. 7.389) and HCO3- (27.4 vs. 22.8 mM) compared with DCAD +200, and metabolic acidosis in DCAD -150 was observed 24 h after dietary treatments started. Concentrations of iCa begin to increase 24 h after feeding the acidogenic diet, and it was greater in DCAD -150 compared with DCAD +200 by 3 d in the experiment (1.23 vs. 1.26 mM)."
Article snippet: Basal concentrations of PTH on d 3 of the experiment were analyzed by ELISA (Bovine IPTH ELISA kit; Creative Diagnostics, New York, NY) in a single assay according to the manufacturer's instructions (http://img2.creative-diagnostics.com/pdf/DEIA1826B.pdf).
Elevated serotonin coordinates mammary metabolism in dairy cows
Meghan K. Connelly, Samantha R. Weaver, Jordan M. Kuehnl, Hannah P. Fricke, Marisa Klister, Laura Hernandez
Physiological Reports2021 AprPubMed ID: 33835711Read Article
Applications: ELISA
Reactive species: Bovine
"Abstract: Serotonin plays a diverse role in maternal and mammary metabolism. Recent research in the dairy cow has shown a relationship between serotonin and calcium, with increased serotonin concentrations improving calcium homeostasis in the peri-partum dairy cow. Therefore, the objective was to elucidate how administration of 5-hydroxy-l-tryptophan (5-HTP), the immediate precursor to serotonin, altered serotonin and calcium metabolism in lactating dairy cows. Twelve mid-late lactation multiparous cows were blocked by parity, production and days in milk and allocated to a daily intravenous infusion of (i) 1.5 mg/kg of 5-HTP (n = 6) or (ii) saline (n = 6) for 3 consecutive days. Milk samples were collected daily. Blood samples were collected before and after each infusion with mammary biopsies and blood samples collected at 48, 56, and 72 h relative to termination of first infusion. Infusion of 5-HTP increased (p = 0.001) circulating serotonin concentrations and decreased blood calcium via a transient hypocalcemia immediately after each infusion (p = 0.02). Treatment with 5-HTP increased milk calcium concentrations (p = 0.02) and calcium release-activated channel protein 1 (ORAI1) mRNA at 56 h and protein at 48 h relative to termination of first infusion (p = 0.008 and p = 0.09, respectively). Fifty-six hours from termination of the first infusion mRNA of parathyroid hormone-related protein and mammary serotonin content were increased relative to control (p = 0.03 and p = 0.05, respectively). These findings demonstrate the ability of 5-HTP infusion to increase circulating serotonin concentrations and alter endocrine and mammary autocrine/paracrine calcium and serotonin metabolism in the lactating dairy cow."
Article snippet: Parathyroid hormone (PTH) concentrations were analyzed in plasma using Bovine IPTH (Intact PTH) ELISA Kit (DEIA1826B, Creative Diagnostics). 
Figure 1. Whole blood serotonin (a), serum total calcium (b), and plasma parathyroid hormone (c) concentrations in multiparous Holstein dairy cows treated with 5-HTP or saline for three days.
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