The IKK-gamma Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor IKK-gamma protein expression profile in cells. The kit can be used for measuring the relative amounts of IKK-gamma in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on IKK-gamma.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 1 plate 2. 10x TBS: 24 mL (10x), Clear 3. Quenching Buffer: 24 mL (1x), Clear 4. Blocking Buffer: 50 mL (1x), Clear 5. 10x Wash Buffer: 50 mL (10x), Clear 6. 100x Anti-IKK-gamma Antibody (Rabbit Polyclonal): 60 μL (100x), Purple 7. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green 8. HRP-Conjugated Anti-Rabbit IgG Antibody: 6 mL (1x), Glass 9. HRP-Conjugated Anti-Mouse IgG Antibody: 6 mL (1x), Glass 10. Primary Antibody Diluent: 12 mL (1x), Clear 11. Ready-to-Use Substrate: 12 mL (1x), Brown 12. Stop Solution: 12 mL (1x), Clear 13. Crystal Violet Solution: 6 mL (1x), Glass 14. SDS Solution: 24 mL (1x), Clear 15. Adhesive Plate Seals: 4 seals
Storage
4°C/6 Months
Citations
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Wang, HY; Gopalan, V; et al. A Custom 148 Gene-Based Resequencing Chip and the SNP Explorer Software: New Tools to Study Antibody Deficiency. HUMAN MUTATION 31:1080-1088(2010).
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