Anti-IKBKE monoclonal antibody (DMABT-H14027)

Mouse anti-Human IKBKE monoclonal antibody for IHC, sELISA, ELISA Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG2b
Clone
2G8
Species Reactivity
Human
Immunogen
IKBKE (NP_054721, 541 a.a. ~ 640 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Unconjugated

Target


Alternative Names
IKBKE; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon; inhibitor of nuclear factor kappa-B kinase subunit epsilon; IKK i; IKKE; KIAA0151
Entrez Gene ID
UniProt ID

Product Background


Gene summary
IKBKE (Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells, Kinase Epsilon) is a Protein Coding gene. Among its related pathways are Immune System and Development EGFR signaling pathway. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is IKBKB. IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al. , 2009 [PubMed 19481526]).
Antigen Description
Serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. Also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling. Following activation of viral RNA sensors, such as RIG-I-like receptors, associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNB. In order to establish such an antiviral state, IKBKE forms several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including IPS1/MAVS, TANK, AZI2/NAP1 or TBKBP1/SINTBAD can be recruited to the IKBKE-containing-complexes. Activated by polyubiquitination in response to TNFA and interleukin-1, regulates the NF-kappa-B signaling pathway through, at least, the phosphorylation of CYLD. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. In addition, is also required for the induction of a subset of ISGs which displays antiviral activity, may be through the phosphorylation of STAT1 at Ser-708. Phosphorylation of STAT1 at Ser-708 seems also to promote the assembly and DNA binding of ISGF3 (STAT1:STAT2:IRF9) complexes compared to GAF (STAT1:STAT1) complexes, in this way regulating the balance between type I and type II IFN responses. Protects cells against DNA damage-induced cell death. Also plays an important role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Phosphorylates AKT1. Inhibitor of nuclear factor kappa-B kinase subunit epsilon also known as I-kappa-B kinase epsilon or IKK-epsilon is an enzyme that in humans is encoded by the IKBKE gene. 0The function about IKBKE antigen include ATP binding; ATP binding; IkappaB kinase activity; NF-kappaB-inducing kinase activity; nucleotide binding; protein binding; protein kinase activity; transferase activity, transferring phosphorus-containing groups.
Pathway
Activated TLR4 signalling, organism-specific biosystem; Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon, organism-specific biosystem; Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; Hepatitis C, organism-specific biosystem; Hepatitis C, conserved biosystem; Herpes simplex infection, organism-specific biosystem.

Citations


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References


Ovsyannikova, I; Haralambieva, I; et al. The role of polymorphisms in Toll-like receptors and their associated intracellular signaling genes in measles vaccine immunity. HUMAN GENETICS 130:547-561(2011).
Nordmark, G; Wang, C; et al. Association of Genes in the NF-kappa B Pathway with Antibody-Positive Primary Sjogren's Syndrome. SCANDINAVIAN JOURNAL OF IMMUNOLOGY 78:447-454(2013).

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