Human IGFBP4 ELISA Kit

Sample

cell culture supernates, cell lysates, tissue homogenates, serum, plasma

Species Reactivity

Human

Intended Use

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IGFBP4 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Contents of Kit

Storage

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were
tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were
tested in separate assays to assess inter-assay precision.

Detection Range

500pg/ml-32000pg/ml

Sensitivity

<20pg/ml

General Description

Insulin-like growth factor-binding protein 4 is a protein that in humans is encoded by the IGFBP4gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. In addition, IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. It is secreted by all colon cancer cells.

Standard Curve