IDO1/IDO2/TDO Inhibitor Screening Assay Kit

Intended Use

The IDO1/IDO2/TDO Inhibitor Screening Assay Kit is designed to measure IDO1, IDO2 and TDO enzyme inhibition.

Contents of Kit

1. IDO1 His-Tag: 20 μg, -80°C;
2. IDO1 Reaction Solution: 10 ml, -80°C;
3. 1x IDO1 Assay Buffer: 1 ml, -80°C;
4. TDO, His-tag: 25 μg, -80°C;
5. TDO Reaction Solution: 10 ml, -80°C;
6. 1x TDO Assay Buffer: 1 ml, -80°C;
7. IDO2 His-Tag: 200 μg, -80°C;
8. IDO2 Reaction Solution: 10 ml, -80°C
9. 1x IDO2 Assay Buffer: 1 ml, -80°C;
10. UV transparent 96-well plate: 1, Room Temp.

Storage

At least one year from date of receipt when stored as directed.

General Description

L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3-dioxygenases (IDO1 & IDO2) and Tryptophan 2,3 dioxygenase (TDO) are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.