Combined Airway Transcriptome Analysis and Immune Proteome Profiling of the Lung Allograft Identifies a Type-1 Immune Signature and Indoleamine 2,3-Dioxygenase 1 (IDO1) as a Potential Biomarker of Chronic Lung Allograft Dysfunction
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
Authors: Wei, J.; Hoji, A.; Iasella, C.; Ferguson, A.; Jain, N.; Zhang, Y.; Brown, M.; Lendermon, E. A.; Johnson, B.; Kilaru, S. D.; Pilewski, J. M.; Morrell, M. R.; Chen, W.; Chen, K.; McDyer, J. F.
Abstract
MiR-218 produces anti-tumor effects on cervical cancer cells in vitro
WORLD JOURNAL OF SURGICAL ONCOLOGY
Authors: Zhu, Li; Tu, Huaidong; Liang, Yanmei; Tang, Dihong
Abstract
Background: As indoleamine-2,3-dioxygenase 1 (IDO1) is critical in tumor immune escape, we determined to study the regulatory mechanism of miR-218 on IDO1 in cervical cancer. Methods: Real-time PCR (RT-qPCR) was carried out to measure the expression of miR-218. RT-qPCR and Western blot were performed to detect the expression of IDO1 in cervical cancer. Dual-luciferase reporter assay was used to determine the binding of miR-218 on the IDO1 3UTR. Cell viability, apoptosis, and related factors were determined using cell counting kit-8 (CCK-8), Annexin-V/PI (propidium) assay, enzyme-linked immunosorbnent assay (ELISA), RT-qPCR, and Western blot assays after miR-218 mimics has been transfected to HeLa cervical cancer cells. Results: MiR-218 was downregulated in cervical cancer. The expression of miR-218 was negatively correlated with IDO1 in cervical cancer tissues and cells. IDO1 is a direct target of miR-218. MiR-218 overexpression was found to inhibit cell viability and promoted apoptosis via activating the expression of Cleaved-Caspase-3 and to inhibit the expression of Survivin, immune factors (TGF-, VEGF, IL-6, PGE2, COX-2), and JAK2/STAT3 pathway. Conclusion: MiR-218 inhibits immune escape of cervical cancer cells by direct downregulating IDO1.
COA
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