Human TDP43 (Tar DNA Binding Protein 43KDa) ELISA Kit

Sample

Serum, plasma, cell culture supernatant and other biological samples

Species Reactivity

Human

Detection Method

sELISA

Intended Use

In vitro quantitative determination of TDP43 concentrations in serum, plasma, cell culture supernatant and other biological samples.

Contents of Kit

1. ELISA Microplate (Dismountable): 8×12, 2-8°C/-20°C. Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C.
2. Lyophilized Standard: 2 vials, 2-8°C/-20°C. Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C
3. Biotin-labeled Antibody (Concentrated): 120 μl, 2-8°C (Avoid Direct Light)
4. HRP-Streptavidin Conjugate (SABC, 100×): 120 μl, 2-8°C (Avoid Direct Light)
5. TMB Substrate: 10 ml, 2-8°C (Avoid Direct Light)
6. Sample Dilution Buffer: 20 ml, 2-8°C
7. Antibody Dilution Buffer: 10 ml, 2-8°C
8. SABC Dilution Buffer: 10 ml, 2-8°C
9. Stop Solution: 10 ml, 2-8°C
10. Wash Buffer (25X): 30 ml, 2-8°C
11. Plate Sealer: 5 pieces
12. Product Description: 1 copy

Storage

Detection Range

0.313 - 20 ng/ml

Sensitivity

0.188 ng/ml

General Description

Uniprot ID: Q13148
Tar DNA Binding Protein 43KDa (TDP-43) is a nuclear RNA-binding protein involved in RNA processing, splicing, and transport. It regulates the expression of target genes by binding to RNA transcripts and modulating their stability, localization, and translation. TDP-43 is predominantly localized in the nucleus under normal conditions, but it can shuttle between the nucleus and cytoplasm in response to cellular stress or pathological stimuli. Dysregulation of TDP-43 expression or localization is associated with neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD), where it forms pathological aggregates and disrupts RNA metabolism.