Human TDP43(Tar DNA Binding Protein 43KDa) ELISA Kit

Sample

Serum, plasma, cell culture supernatant, cell lysate or tissue lysate, other biological fluid samples.

Species Reactivity

Human

Detection Method

sELISA

Intended Use

In vitro quantitative determination of TDP43 concentrations in Serum, plasma, cell culture supernatant, cell lysate or tissue lysate, other biological fluid samples.

Contents of Kit

1. ELISA Microplate(Dismountable), 8×12. Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C.
2. Lyophilized Standard, 2 vials. Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C.
3. Cap/Det Ab (Ready to use), 6 ml. 2-8°C (Avoid Direct Light).
4. HRP-Streptavidin (Ready to use, orange), 10 ml. 2-8°C (Avoid Direct Light).
5. TMB Substrate, 10 ml, 2-8°C (Avoid Direct Light)
6. Sample Dilution Buffer (blue), 20 ml, 2-8°C.
7. Stop Solution, 5 ml, 2-8°C.
8. Wash Buffer (25X), 30 ml, 2-8°C.
9. Plate Sealer, 5 pieces.

Storage

2-8°C (for sealed box), please do not freeze! See kit label for expiry date.

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Detection Range

0.313-20 ng/ml

Sensitivity

0.188 ng/ml

General Description

Tar DNA Binding Protein 43KDa (TDP-43) is a nuclear RNA-binding protein involved in RNA processing, splicing, and transport. It regulates the expression of target genes by binding to RNA transcripts and modulating their stability, localization, and translation. TDP-43 is predominantly localized in the nucleus under normal conditions, but it can shuttle between the nucleus and cytoplasm in response to cellular stress or pathological stimuli. Dysregulation of TDP-43 expression or localization is associated with neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD), where it forms pathological aggregates and disrupts RNA metabolism.

General Notes

In vitro quantitative determination of TDP43 concentrations in Serum, plasma, cell culture supernatant, cell lysate or tissue lysate, other biological fluid samples.