Human TDO Inhibitor Screening Assay Kit

Species Reactivity

Human

Intended Use

The TDO Inhibitor Screening Assay Kit is designed to measure TDO enzyme inhibition.

Contents of Kit

1. TDO, His-tag: 50 μg, -80°C ;
2. TDO Reaction Solution: 2 x 10 ml, -80°C;
3. 1x TDO Assay Buffer: 1 ml, -80°C;
4. UV transparent 96-well plate: 1, Room Temp.

Storage

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

General Description

L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Tryptophan 2,3 dioxygenase (TDO) is upregulated in many tumors, providing cancer cells with an avenue for immune evasion.